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MINFLUX nanoscopy: Visualising biological matter at the nanoscale level

Carsten, Alexander ; Failla, Antonio Virgilio ; Aepfelbacher, Martin

Journal of microscopy (Oxford), 2024-04 [Periódico revisado por pares]

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Título:
MINFLUX nanoscopy: Visualising biological matter at the nanoscale level
Autor: Carsten, Alexander ; Failla, Antonio Virgilio ; Aepfelbacher, Martin
É parte de: Journal of microscopy (Oxford), 2024-04
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Abstract Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, fluorescent marker developments and biological studies that have been conducted in connection with MINFLUX imaging and tracking. We also formulate ideas on how MINFLUX nanoscopy and derived technologies could influence bioimaging in the future. This insight is intended as a general starting point for an audience looking for a brief overview of MINFLUX nanoscopy from theory to application. LAY DESCRIPTION MINFLUX microscopy can image fluorescent molecules with great precision. This review gives an overview of MINFLUX microscopy from theory to application, explaining how it works, how it has been used in biological studies and how it could be used in the future.
Idioma: Italiano

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