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Continuous flow separation of particles with insulator-based dielectrophoresis chromatography

Hill, Nicole ; Lapizco-Encinas, Blanca H.

Analytical and bioanalytical chemistry, 2020-06, Vol.412 (16), p.3891-3902 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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  • Título:
    Continuous flow separation of particles with insulator-based dielectrophoresis chromatography
  • Autor: Hill, Nicole ; Lapizco-Encinas, Blanca H.
  • Assuntos: Analytical Chemistry ; Arrays ; Bioanalytics and Higher Order Electrokinetics ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Continuous flow ; Dielectrophoresis ; Experimentation ; Food Science ; Laboratory Medicine ; Mathematical models ; Monitoring/Environmental Analysis ; Polarizability ; Research Paper ; Retention ; Retention time ; Separation ; Stationary phase ; Surface charge
  • É parte de: Analytical and bioanalytical chemistry, 2020-06, Vol.412 (16), p.3891-3902
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: The development of insulator-based dielectrophoresis chromatography is proposed here as a novel hybrid technique that capitalizes on the simplicity of insulator-based dielectrophoresis (iDEP) and the well-known chromatographic theory. Chromatographic parameters are employed to characterize dielectrophoretic separation of particles with particles being eluted from the system as enriched particle peaks . By varying the characteristics of the insulating posts, it was possible to manipulate the interactions of the particles with the insulating post array which acted as the stationary phase . The present work studied how the characteristics of the particles affected the particle retention . Different types of particles have distinct interactions with the post array; these interactions depend on particle properties (size, electrical charge, and polarizability). This work includes mathematical modeling with COMSOL and extensive experimentation. Particles ranging from 1 to 10 μm in diameter were tested for retention time and eluted as peaks in the iDEP chromatography devices. Separation results were reported in the form of dielectropherograms including the estimation of retention time ( t R ), separation efficiency ( N /meter), and separation resolution ( R s ). Two full separations were demonstrated: a separation by charge between two types of particles of similar size (~ 10 μm) with different electrical surface charges and a separation by size between 2- and 5-μm particles with similar surface charge (difference in ζ P of 4 mV). The achieved separation resolutions were R s  = 1.8 and R s  = 3.5, respectively. This is the first study on DEP chromatography to assess performance in terms of resolution and separation efficiency, demonstrating the unique potential of iDEP chromatography.
  • Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
  • Idioma: Inglês
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