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Microfluidic devices for size-dependent separation of liver cells

Yamada, Masumi ; Kano, Kyoko ; Tsuda, Yukiko ; Kobayashi, Jun ; Yamato, Masayuki ; Seki, Minoru ; Okano, Teruo

Biomedical microdevices, 2007-10, Vol.9 (5), p.637-645 [Periódico revisado por pares]

United States: Springer Nature B.V

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Título:
Microfluidic devices for size-dependent separation of liver cells
Autor: Yamada, Masumi ; Kano, Kyoko ; Tsuda, Yukiko ; Kobayashi, Jun ; Yamato, Masayuki ; Seki, Minoru ; Okano, Teruo
Assuntos: Animals ; Benzimidazoles - metabolism ; Biochemistry ; Cell Nucleus - metabolism ; Cell Separation - instrumentation ; Cell Separation - methods ; Cell Size ; Cell Survival - drug effects ; Cells ; Cells, Cultured ; Collagenases - pharmacology ; Dimethylpolysiloxanes - chemistry ; Equipment Design ; Fluorescein-5-isothiocyanate ; Fluorescent Antibody Technique, Indirect ; Fluorescent Dyes ; Glass - chemistry ; Green Fluorescent Proteins - metabolism ; Hepatocytes - cytology ; Liver ; Liver - cytology ; Male ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Perfusion ; Polymers - chemistry ; Rats ; Rats, Inbred Lew ; Rats, Wistar ; Scientific apparatus & instruments
É parte de: Biomedical microdevices, 2007-10, Vol.9 (5), p.637-645
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Descrição: Liver is composed of various kinds of cells, including hepatic parenchymal cells (hepatocytes) and nonparenchymal cells, and separation of these cells is essential for cellular therapies and pharmacological and metabolic studies. Here, we present microfluidic devices for purely hydrodynamic and size-dependent separation of liver cells, which utilize hydrodynamic filtration. By continuously introducing cell suspension into a microchannel with multiple side-branch channels, cells smaller than a specific size are removed from the mainstream, while large cells are focused onto a sidewall in the microchannel and then separated into two or three groups. Two types of PDMS-glass hybrid microdevices were fabricated, and rat liver cells were successfully separated. Also, cell size, morphology, viability and several cell functions were analyzed, and the separation performances of the microfluidic devices were compared to that of a conventional centrifugal technique. The results showed that the presented microfluidic devices are low-cost and suitable for clinical use, and capable of highly functional separation with relatively high-speed processing.
Editor: United States: Springer Nature B.V
Idioma: Inglês

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Microfluidic devices for size-dependent separation of liver cells

Yamada, Masumi ; Kano, Kyoko ; Tsuda, Yukiko ; Kobayashi, Jun ; Yamato, Masayuki ; Seki, Minoru ; Okano, Teruo

United States: Springer Nature B.V

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