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Diversity of Cultivated Fungi Associated with Conventional and Transgenic Sugarcane and the Interaction between Endophytic Trichoderma virens and the Host Plant

Romão-Dumaresq, Aline Silva ; Dourado, Manuella Nóbrega ; Fávaro, Léia Cecilia de Lima ; Mendes, Rodrigo ; Ferreira, Anderson ; Araújo, Welington Luiz Acharya, Krishnendu

PloS one, 2016-07, Vol.11 (7), p.e0158974-e0158974 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Diversity of Cultivated Fungi Associated with Conventional and Transgenic Sugarcane and the Interaction between Endophytic Trichoderma virens and the Host Plant
Autor: Romão-Dumaresq, Aline Silva ; Dourado, Manuella Nóbrega ; Fávaro, Léia Cecilia de Lima ; Mendes, Rodrigo ; Ferreira, Anderson ; Araújo, Welington Luiz
Acharya, Krishnendu
Assuntos: Ascomycota ; Biodiversity ; Biological control ; Biology and Life Sciences ; Cellulase ; Communities ; Crop management ; Crop Production ; Crops ; Diseases and pests ; DNA, Fungal - genetics ; Ecology and Environmental Sciences ; Ecosystem biology ; Ecosystems ; Endophytes ; Endophytes - physiology ; Epidermis ; Ethanol ; Fluorescence ; Fungi ; Fungi, Phytopathogenic ; Genetic modification ; Genetically modified organisms ; Green fluorescent protein ; Growth stage ; Herbicide resistance ; Herbicide Resistance - genetics ; Herbicides ; Host plants ; Hypocrea virens ; Laboratories ; Lignocellulose ; Medicine and Health Sciences ; Microorganisms ; Molecular biology ; Mycelia ; Phylogeny ; Physiological aspects ; Phytopathogenic fungi ; Plant communities ; Plant growth ; Plant protection ; Plant roots ; Plant Roots - microbiology ; Plant Roots - physiology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - microbiology ; Plants, Genetically Modified - physiology ; Rhizosphere ; Roots ; Saccharum - genetics ; Saccharum - microbiology ; Saccharum - physiology ; Saccharum officinarum ; Sequence Analysis, DNA ; Studies ; Sugarcane ; Symbiosis - genetics ; Symbiosis - physiology ; Transgenic plants ; Trichoderma ; Trichoderma - genetics ; Trichoderma - physiology ; Trichoderma harzianum ; Trichoderma virens ; Very Low Frequencies
É parte de: PloS one, 2016-07, Vol.11 (7), p.e0158974-e0158974
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: ASR RM LCLF WLA. Performed the experiments: ASR LCLF AF. Analyzed the data: ASR MND. Contributed reagents/materials/analysis tools: ASR MND WLA. Wrote the paper: ASR MND WLA.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants. Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance). For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). In addition, a strain of Trichoderma virens, an endophyte isolated from sugarcane with great potential as a biological control, growth promotion and enzyme production agent, was selected for the fungal-plant interaction assays. The results of the isolation, characterization and evaluation of fungal community changes showed that the sugarcane fungal community is composed of at least 35 different genera, mostly in the phylum Ascomycota. Many genera are observed at very low frequencies among a few most abundant genera, some of which were isolated from specific plant sites (e.g., the roots or the rhizosphere). An assessment of the possible effects upon the fungal community showed that the plant growth stage was the only factor that significantly affected the community's structure. Moreover, if transgenic effects are present, they may be minor compared to other natural sources of variation. The results of interaction studies using the Green fluorescent protein (GFP)-expressing T. virens strain T.v.223 revealed that this fungus did not promote any phenotypic changes in the host plant and was found mostly in the roots where it formed a dense mycelial cover and was able to penetrate the intercellular spaces of the root epidermis upper layers. The ability of T. virens to colonize plant roots suggests a potential for protecting plant health, inhibiting pathogens or inducing systemic resistance.
Editor: United States: Public Library of Science
Idioma: Inglês

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Diversity of Cultivated Fungi Associated with Conventional and Transgenic Sugarcane and the Interaction between Endophytic Trichoderma virens and the Host Plant

Romão-Dumaresq, Aline Silva ; Dourado, Manuella Nóbrega ; Fávaro, Léia Cecilia de Lima ; Mendes, Rodrigo ; Ferreira, Anderson ; Araújo, Welington Luiz Acharya, Krishnendu

United States: Public Library of Science

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