Idioma:

Transformation of Kluyveromyces lactis by electroporation

Sanchez, M ; Iglesias, F.J ; Santamaria, C ; Dominguez, A

Applied and Environmental Microbiology, 1993-07, Vol.59 (7), p.2087-2092 [Periódico revisado por pares]

Washington, DC: American Society for Microbiology

Texto completo disponível

Citações Citado por

Enviar para

Título:
Transformation of Kluyveromyces lactis by electroporation
Autor: Sanchez, M ; Iglesias, F.J ; Santamaria, C ; Dominguez, A
Assuntos: Bacteria ; Biological and medical sciences ; Biotechnology ; Cellular biology ; Electricity ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Kluyveromyces lactis ; KLUYVEROMYCES MARXIANUS ; Methods. Procedures. Technologies ; Miscellaneous ; Mycology ; Synthetic digonucleotides and genes. Sequencing ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Yeast
É parte de: Applied and Environmental Microbiology, 1993-07, Vol.59 (7), p.2087-2092
Notas: 9426451
F30
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Corresponding author.
Descrição: The physical and biological parameters involved in efficient transformation of Kluyveromyces lactis by electroporation have been analyzed. By using an optimum voltage and a constant volume of cell suspension in a cuvette, the efficiency of transformation increased with increases in cell numbers and plasmid concentration. However, the most important parameter was the time of the pulse. Changes of 1 ms decreased the efficiency of transformation more than 70 to 80%. Under our best conditions, between 10(7) and transformants per microgram of plasmid DNA could be obtained. Under certain conditions, the size of the plasmid also affected electroporation efficiency. In any case, we did not obtain integrative transformation with an autonomously replicating plasmid
Editor: Washington, DC: American Society for Microbiology
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Transformation of Kluyveromyces lactis by electroporation

Sanchez, M ; Iglesias, F.J ; Santamaria, C ; Dominguez, A

Washington, DC: American Society for Microbiology

Buscando em bases de dados remotas. Favor aguardar.