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Polarization microscopy as a tool for quantitative evaluation of collagen using picrosirius red in different stages of CKD in cats

Morais, G. B. ; Viana, D. A. ; Silva, F. M. O. ; Xavier Júnior, F. A. F. ; Farias, K. M. ; Pessoa, C. D'Ó ; Silveira, J. A. M. ; Alves, A. P. N. N. ; Mota, M. R. L. ; Silva, F. D. O. ; Sampaio, C. M. S. ; Verdugo, J. M. G. ; Evangelista, J. S. A. M.

Microscopy research and technique, 2017-05, Vol.80 (5), p.543-550 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
Polarization microscopy as a tool for quantitative evaluation of collagen using picrosirius red in different stages of CKD in cats
Autor: Morais, G. B. ; Viana, D. A. ; Silva, F. M. O. ; Xavier Júnior, F. A. F. ; Farias, K. M. ; Pessoa, C. D'Ó ; Silveira, J. A. M. ; Alves, A. P. N. N. ; Mota, M. R. L. ; Silva, F. D. O. ; Sampaio, C. M. S. ; Verdugo, J. M. G. ; Evangelista, J. S. A. M.
Assuntos: Animals ; Azo Compounds - chemistry ; Cat Diseases - diagnosis ; Cat Diseases - pathology ; Cats ; chronic kidney disease ; Collagen ; Collagen - analysis ; Collagen - ultrastructure ; Collagens ; Coloring Agents - chemistry ; Correlation ; Creatinine - blood ; Damage ; Deposition ; feline ; Female ; Fibrosis ; Interstitials ; kidney ; Kidney - chemistry ; Kidney - pathology ; Kidney - ultrastructure ; Kidneys ; Male ; Microscopy ; Microscopy, Polarization - methods ; picrosirius red ; Renal Insufficiency, Chronic - diagnosis ; Renal Insufficiency, Chronic - pathology ; Renal Insufficiency, Chronic - veterinary ; Severity of Illness Index
É parte de: Microscopy research and technique, 2017-05, Vol.80 (5), p.543-550
Notas: Review Editor: Prof. Alberto Diaspro
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SourceType-Scholarly Journals-1
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Descrição: Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization. Statistics correlations were performed by Spearman's (ρ; p < .05). There was a significant correlation of IF quantification with the area of interstitial collagen deposition by polarized PSR (PSRp) (r = .7939, p = .0098) and nonpolarized PSR (PSRn) (r = .7781, p = .0080). There was a positive correlation of serum creatinine (sCr) at different stages of CKD with PSRp (r = .7939, p = .0098), PSRn (r = .8667, p = .0027) and MT (r = .7818, p = .0117). Correlations between the percentage of quantified area was also positive from PSRp to PSRn (r = .9030, p = .0009) and PSRp to MT (r = .7939, p = .0098). The PSRN was also correlated with MT (r = .9273, p = .0001). The correlation with IF and sCr follows the disease evolution and the quantification of collagen by PSR is an excellent tool for analyzing the disease severity at different stages. The quantification of interstitial collagen is an excellent tool to analyze tubulointerstitial damage in cats with CKD Circular polarization microscopy is a good apparatus for the analysis of collagen deposition in kidneys of cats with CKD.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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Polarization microscopy as a tool for quantitative evaluation of collagen using picrosirius red in different stages of CKD in cats

Morais, G. B. ; Viana, D. A. ; Silva, F. M. O. ; Xavier Júnior, F. A. F. ; Farias, K. M. ; Pessoa, C. D'Ó ; Silveira, J. A. M. ; Alves, A. P. N. N. ; Mota, M. R. L. ; Silva, F. D. O. ; Sampaio, C. M. S. ; Verdugo, J. M. G. ; Evangelista, J. S. A. M.

United States: Wiley Subscription Services, Inc

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