Language:

Isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (Panicum virgatum L.) cultivars

Gagne‐Bourgue, F. ; Aliferis, K.A. ; Seguin, P. ; Rani, M. ; Samson, R. ; Jabaji, S.

Journal of applied microbiology, 2013-03, Vol.114 (3), p.836-853 [Peer Reviewed Journal]

Oxford: Blackwell

Full text available

Citations Cited by

Actions
1. Add to e-Shelf
2. Remove from e-Shelf
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (Panicum virgatum L.) cultivars
Author: Gagne‐Bourgue, F. ; Aliferis, K.A. ; Seguin, P. ; Rani, M. ; Samson, R. ; Jabaji, S.
Subjects: Bacillus ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; bioenergy grass ; Biological and medical sciences ; biologically active metabolites ; Cultivars ; DNA Fingerprinting ; DNA, Bacterial - genetics ; endophytes ; Endophytes - classification ; Endophytes - genetics ; Endophytes - isolation & purification ; Environmental Microbiology ; Fundamental and applied biological sciences. Psychology ; Fungi ; Microbacterium ; Microbiology ; molecular detection ; Panicum - microbiology ; Panicum virgatum ; Phylogeny ; phytohormones ; Plant Leaves - microbiology ; RNA, Ribosomal, 16S - genetics ; Seeds ; Seeds - microbiology ; toxins ; vertical transmission ; Volatile Organic Compounds - chemistry
Is Part Of: Journal of applied microbiology, 2013-03, Vol.114 (3), p.836-853
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Aims To isolate and characterize indigenous bacterial endophytes from cultivars of switchgrass and study their antimicrobial and growth promoting potential. Methods and Results The diversity, molecular and biochemical characterizations of indigenous and culturable bacterial endophytes residing in leaves of switchgrass have not been studied previously. This study describes the characterization of 31 bacterial endophytes from three switchgrass cutlivars: Cave‐in Rock, Blue Jacket and Tecumseh. Molecular and phylogenetic analysis based on the 16S rRNA sequence grouped the endophytes into eight different taxa that shared high homology of 98–99% with other known sequences. Bacterial endophytes were identified as Microbacterium testaceum, Curtobacterium flaccumfaciens, Bacillus subtilis and Bacillus pumilus, Pseudomonas fluorescens, Sphingomonas parapaucimobilis, Serratia sp. and Pantoea ananatis. Some endophytes were detected in switchgrass seeds and in plants that originated from seeds collected a year earlier, confirming vertical transmission to the next generation of the host. Selected endophytes produced cellulases and were capable of solubilizing inorganic phosphorus. Analysis of cell‐free culture filtrate of selected strains using direct infusion orbitrap mass spectrometry confirmed the presence of several well‐characterized lipopeptide toxins and phytohormones. Re‐inoculation of the roots of switchgrass seedlings with endophytes singly or combined confirmed their migration to the upper aerial parts of the plant. Conclusions Our findings suggest that switchgrass leaves harbour a diversity of bacterial endophytes, some of which could potentially be applied as growth promoting bacteria. Significance and Impact of the Study This is the first report on the characterization of indigenous bacterial endophytes and their potential use as biofertilizers.
Publisher: Oxford: Blackwell
Language: English

Links

Back to results list

Previous Result 13 Next Go to Next page

Implemented by: Social Network Logos:

Isolation and characterization of indigenous endophytic bacteria associated with leaves of switchgrass (Panicum virgatum L.) cultivars

Gagne‐Bourgue, F. ; Aliferis, K.A. ; Seguin, P. ; Rani, M. ; Samson, R. ; Jabaji, S.

Oxford: Blackwell

Searching Remote Databases, Please Wait