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The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma

Lei, Hua ; Gui, Lai ; Xiao, Ran

Clinical biochemistry, 2009-09, Vol.42 (13), p.1452-1460 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma
Autor: Lei, Hua ; Gui, Lai ; Xiao, Ran
Assuntos: Adenosine - pharmacology ; Adolescent ; Adult ; Anticoagulant ; Anticoagulants - pharmacology ; Biological effect ; Blood Platelets - drug effects ; Blood Platelets - ultrastructure ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Citric Acid - pharmacology ; Dipyridamole - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Female ; Glucose - analogs & derivatives ; Glucose - pharmacology ; Growth factor ; Heparin - pharmacology ; Humans ; Male ; Microscopy, Electron, Transmission ; P-Selectin - blood ; Platelet activation ; Platelet-rich plasma ; Platelet-Rich Plasma - drug effects ; Platelet-Rich Plasma - metabolism ; Stromal Cells - cytology ; Stromal Cells - drug effects ; Theophylline - pharmacology ; Transforming Growth Factor beta1 - blood ; Young Adult
É parte de: Clinical biochemistry, 2009-09, Vol.42 (13), p.1452-1460
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: This study investigated the effect of anticoagulants on platelet-rich plasma (PRP) quality to determine the appropriate anticoagulants for PRP production. This study was carried out at the Plastic Surgery Hospital of Peking Union Medical College. The microstructure of platelets collected with heparin, citrate, acid citrate dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole (CTAD) was observed. The extent of spontaneous activation of platelets was detected by measuring sP-selectin in plasma. The amount of TGF-β1 released from PRP and the effect of PRP on cell proliferation were also studied. ACD and CTAD were superior to heparin and citrate in maintaining the integrity of platelet structures and preventing the platelet spontaneous activation. ACD-PRP and CTAD-PRP released more TGF-β1 and significantly enhanced the proliferation of human marrow stromal cells compared to heparin-PRP and citrate-PRP. The PRP quality was closely related to the type of anticoagulants. ACD and CTAD are appropriate anticoagulants for PRP production.
Editor: United States: Elsevier Inc
Idioma: Inglês

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The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma

Lei, Hua ; Gui, Lai ; Xiao, Ran

United States: Elsevier Inc

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