skip to main content
Idioma:

Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement

Barreto, Rodrigo da Silva Nunes ; Romagnolli, Patricia ; Mess, Andrea Maria ; Miglino, Maria Angelica

Journal of tissue engineering and regenerative medicine, 2018-04, Vol.12 (4), p.e1880-e1888 [Periódico revisado por pares]

England: Hindawi Limited

Texto completo disponível

Citações Citado por
  • Título:
    Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement
  • Autor: Barreto, Rodrigo da Silva Nunes ; Romagnolli, Patricia ; Mess, Andrea Maria ; Miglino, Maria Angelica
  • Assuntos: Animal models ; Blood vessels ; cattle ; Collagen ; Collagen (type I) ; Collagen (type IV) ; Cotyledons ; Deoxyribonucleic acid ; DNA ; DNA structure ; Electron microscopy ; Extracellular matrix ; Fetuses ; Fibers ; Fibronectin ; Histology ; Immunohistochemistry ; Laminin ; Mesenchyme ; Organs ; Perfusion ; placenta ; Proteins ; Regenerative medicine ; Scaffolds ; Sodium ; Sodium dodecyl sulfate ; Sodium lauryl sulfate ; Tissue engineering ; Triton
  • É parte de: Journal of tissue engineering and regenerative medicine, 2018-04, Vol.12 (4), p.e1880-e1888
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Technically produced scaffolds are common to establish transplantable tissues for regenerative medicine, but also biological ones that are closer to the natural condition become of interest. Placentas are promising, because they represented available, complete organs with rich extracellular matrix (ECM) and well‐developed vasculature that easily could build anastomoses to a host's organ. Only placentas from larger animal models such as the bovine meet the dimensions large enough for most organs but are not adequately described yet. We here studied the nature of the ECM in 27 natural and decellularized bovine cotyledons, that is, the fetal part of the placentomes, by means of histology, immunohistochemistry, and electron microscopy. Successful decellularization was done by perfusion with 0.01%, 0.1%, and 0.5% sodium dodecyl sulfate each and subsequent immersion in 1% Triton X‐100, resulting in a removal of cells and DNA, whereas the structure of the allantochorionic surface and villi was preserved. Although some fibres disappeared, also the arrangement of the main ECM proteins was largely similar before and after decellularization: Along the larger vessels, a densely packed network of thick fibres occurred, organized in layers without cells or spaces in between. Collagen IV, fibronectin, and laminin contributed to those areas. In contrast, collagen I and III characterized the meshwork of medium‐sized and thin fibres in the mesenchyme, respectively. In conclusion, decellularized bovine cotyledons indeed had characteristics of a biological scaffold and provide an interesting alternative to develop large‐scale scaffolds with complex vascular architecture for tissue engineering purposes.
  • Editor: England: Hindawi Limited
  • Idioma: Inglês
Links
Voltar para lista de resultados
Resultado  1 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.