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Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny

Calaminus, Simon D J ; Guitart, Amelie V ; Guitart, Amelie ; Sinclair, Amy ; Schachtner, Hannah ; Watson, Steve P ; Holyoake, Tessa L ; Kranc, Kamil R ; Machesky, Laura M Freson, Kathleen

PloS one, 2012-12, Vol.7 (12), p.e51361-e51361 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny
Autor: Calaminus, Simon D J ; Guitart, Amelie V ; Guitart, Amelie ; Sinclair, Amy ; Schachtner, Hannah ; Watson, Steve P ; Holyoake, Tessa L ; Kranc, Kamil R ; Machesky, Laura M
Freson, Kathleen
Assuntos: Analysis ; Animals ; B cells ; Biology ; Blood platelets ; Blood Platelets - metabolism ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Cancer ; Cell Lineage ; Cells (biology) ; Cells, Cultured ; DNA - genetics ; Fetus - cytology ; Fetus - metabolism ; Fetuses ; Flow Cytometry ; Gene expression ; Hematology ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - metabolism ; Human health and pathology ; Integrases - metabolism ; Journalistic ethics ; Kinases ; Leukemia ; Life Sciences ; Liver ; Liver - cytology ; Liver - metabolism ; Lymphocytes - cytology ; Lymphocytes - metabolism ; Medical research ; Medicine ; Megakaryocytes - cytology ; Megakaryocytes - metabolism ; Mice ; Mice, Transgenic ; Myeloid Cells - cytology ; Myeloid Cells - metabolism ; Platelet Factor 4 - physiology ; Progeny ; Real-Time Polymerase Chain Reaction ; Rodents ; Stem cell transplantation ; Stem cells ; Studies ; Transgenic animals
É parte de: PloS one, 2012-12, Vol.7 (12), p.e51361-e51361
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
These authors also contributed equally to this work.
Conceived and designed the experiments: KRK LM. Performed the experiments: SDJC AS AG HS. Analyzed the data: SDJC AS AG HS. Wrote the paper: SDJC AS AG SPW TLH KRK LMM.
Descrição: The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.
Editor: United States: Public Library of Science
Idioma: Inglês

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Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny

Calaminus, Simon D J ; Guitart, Amelie V ; Guitart, Amelie ; Sinclair, Amy ; Schachtner, Hannah ; Watson, Steve P ; Holyoake, Tessa L ; Kranc, Kamil R ; Machesky, Laura M Freson, Kathleen

United States: Public Library of Science

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