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Phylogenetic analysis of South American sequences of the nonstructural protein-1 (ns1) of dengue serotype 2 associated with severe clinical bleeding

Castro-Orozco, Raimundo ; Castro-García, Lyda R ; Gómez-Camargo, Doris E

Revista de salud pública (Bogotá, Colombia), 2016-06, Vol.18 (3), p.459-469 [Periódico revisado por pares]

Colombia

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  • Título:
    Phylogenetic analysis of South American sequences of the nonstructural protein-1 (ns1) of dengue serotype 2 associated with severe clinical bleeding
  • Autor: Castro-Orozco, Raimundo ; Castro-García, Lyda R ; Gómez-Camargo, Doris E
  • Assuntos: Amino Acid Sequence ; Base Sequence ; Bayes Theorem ; Dengue Virus - genetics ; Hemorrhage - virology ; Humans ; Phylogeny ; Serogroup ; Severe Dengue - complications ; Severe Dengue - virology ; Viral Nonstructural Proteins - classification ; Viral Nonstructural Proteins - genetics
  • É parte de: Revista de salud pública (Bogotá, Colombia), 2016-06, Vol.18 (3), p.459-469
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Objective The objective of this in silico study was to compare nucleotide and amino acids DENV-2-NS1 sequences isolated from febrile patients, with and without disease severity, from different South American countries. Matherials and Methods A bayesian MCMC phylogenetic analysis was carried out using 28 complete sequences of the gene NS1 of the DENV-2 serotype (1 056 bp), using MrBayes v.3.2.0 software, with the model SYM+G (2.5 million generations). We also carried out a phylogenetic analysis with Neighbor-Joining method (Jukes-Cantor model). In addition, the amino acids sequences were aligned and compared with each other, using Clustal W included in MEGA v.5.2 software. Results In the amino acids sequences associated with bleeding, the most frequent substitution was isoleucine → threonine at posicion 93. These sequences showed a high percentage (94.6 %) of amino acid homology in comparison with the percentage of amino acids homology (74 %) of DENV-2 isolates not associated with bleeding. Five clades were identified that group the vast majority of the DENV-2-NS1 sequences analyzed (19/24; 79.2 %) with posterior probability values greater than or equal to 58 %. Seven sequences (87.5 %) associated with bleeding were phylogenetically related within clades 4 and 5, the posterior probability values were 58 % and 97 %, respectively. Conclusion Neither phylogenetic characteristics nor differences between amino acids of the DENV-2-NS1 sequences studied were found that could be associated directly with severity of the disease.
  • Editor: Colombia
  • Idioma: Espanhol
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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