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A recurrent mutation at position 1 26,340 of SARS-CoV-2 is associated with failure of the E-gene qRT-PCR utilized in a commercial dual-target diagnostic assay

Artesi, Maria ; Bontems, Sébastien ; Göbbels, Paul ; Franckh, M ; Maes, Piet ; BOREUX, Raphaël ; MEEX, Cécile ; MELIN, Pierrette ; Hayette, Marie-Pierre ; Bours, Vincent ; Durkin, Keith

American Society for Microbiology 2020

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  • Título:
    A recurrent mutation at position 1 26,340 of SARS-CoV-2 is associated with failure of the E-gene qRT-PCR utilized in a commercial dual-target diagnostic assay
  • Autor: Artesi, Maria ; Bontems, Sébastien ; Göbbels, Paul ; Franckh, M ; Maes, Piet ; BOREUX, Raphaël ; MEEX, Cécile ; MELIN, Pierrette ; Hayette, Marie-Pierre ; Bours, Vincent ; Durkin, Keith
  • Assuntos: COVID ; dual-target ; E GENE ; Human health sciences ; Laboratory medicine & medical technology ; Médecine de laboratoire & technologie médicale ; PCR ; SARS-CoV-2 ; Sciences de la santé humaine
  • Notas: scopus-id:2-s2.0-85091594563
  • Descrição: Control of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic requires accurate laboratory testing to identify infected individuals, while also clearing essential staff to continue work. At the current time a number of qRT-PCR assays have been developed to identify SARS-CoV-2, targeting multiple positions in the viral genome. While the mutation rate of SARS-CoV-2 is moderate, given the large number of transmission chains it is prudent to monitor circulating viruses for variants that might compromise these assays. Here we report the identification of a C-to-U transition at position 26,340 of the SARS-CoV-2 genome which is associated with failure of the cobas® SARS-CoV-2 E-gene qRT-PCR in eight patients. As the cobas® SARS-CoV-2 assay targets two positions in the genome, the individuals carrying this variant were still called as SARS-CoV-2 positive. Whole genome sequencing of SARS-CoV-2 showed all to carry closely related viruses. Examination of viral genomes deposited on GISAID showed this mutation has arisen independently at least four times. This work highlights the necessity of monitoring SARS-CoV-2 for the emergence of SNPs which might adversely affect RT PCRs used in diagnostics. Additionally, it argues that two regions in SARS-CoV-2 should be targeted to avoid false negatives.
  • Editor: American Society for Microbiology
  • Data de criação/publicação: 2020
  • Idioma: Inglês
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