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Identification of DNA amplification fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.)

Men, A.E ; Borisov, A.Y ; Rozov, S.M ; Ushakov, K.V ; Tsyganov, V.E ; Tikhonovich, I.A ; Gresshoff, P.M

Theoretical and applied genetics, 1999-05, Vol.98 (6/7), p.929-936 [Periódico revisado por pares]

Heidelberg: Springer

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Título:
Identification of DNA amplification fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.)
Autor: Men, A.E ; Borisov, A.Y ; Rozov, S.M ; Ushakov, K.V ; Tsyganov, V.E ; Tikhonovich, I.A ; Gresshoff, P.M
Assuntos: Bacterial infections ; biochemical techniques ; bulked segregant analysis ; chromosome mapping ; Cloning ; DNA fingerprinting ; genbank/af042741 ; genbank/af042742 ; Genes ; genetic markers ; Genomes ; Genotype & phenotype ; Legumes ; loci ; molecular sequence data ; Mutation ; nodulation ; nucleotide sequences ; Pisum sativum ; polymerase chain reaction ; Rhizobium leguminosarum ; Seeds ; sequence characterized amplified region ; sym31 gene ; sym31 locus ; symbiosis
É parte de: Theoretical and applied genetics, 1999-05, Vol.98 (6/7), p.929-936
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: We demonstrate efficient genome mapping through a combination of bulked segregant analysis (BSA) with DNA amplification fingerprinting (DAF). Two sets of 64 octamer DAF primers, along with two PCR programs of low- and high-annealing temperatures (30 degrees C and 55 degrees C, respectively), appeared to be enough to locate molecular markers within 2-5 cM of a gene of interest. This approach allowed the rapid identification of four BSA markers linked to the pea (Pisum sativum L.) Sym31 gene, which is responsible for bacteroid and symbiosome differentiation. Three of these markers are shown to be tightly linked to the sym31 mutation. Two markers flanking the Sym31 gene, A21-310 and B1-277, cover a 4-5 cM interval of pea linkage group 3. Both markers were converted to sequence-characterized amplified regions (SCARs). The flanking markers may be potential tools for marker-assisted selection or for positional cloning of the Sym31 gene.
Editor: Heidelberg: Springer
Idioma: Inglês;Russo

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Identification of DNA amplification fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.)

Men, A.E ; Borisov, A.Y ; Rozov, S.M ; Ushakov, K.V ; Tsyganov, V.E ; Tikhonovich, I.A ; Gresshoff, P.M

Heidelberg: Springer

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