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Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus)

Marie-Odile Monier Chelini Nívea Lopes de Souza; Cláudio Alvarenga de Oliveira; A. M Rocha; Érika Cristiane Gutierrez Felippe

Brazilian Journal of Medical and Biological Research Ribeirão Preto v. 38, n. 11, p. 1711-1717, 2005

Ribeirão Preto 2005

Localização: FMVZ - Fac. Med. Vet. e Zootecnia    (SOU - 35 )(Acessar)

  • Título:
    Quantification of fecal estradiol and progesterone metabolites in syrian hamsters (Mesocricetus auratus)
  • Autor: Marie-Odile Monier Chelini
  • Nívea Lopes de Souza; Cláudio Alvarenga de Oliveira; A. M Rocha; Érika Cristiane Gutierrez Felippe
  • Assuntos: HAMSTERS; ENDOCRINOLOGIA VETERINÁRIA; ESTRÓGENOS; RADIOIMUNOENSAIO; REPRODUÇÃO ANIMAL
  • É parte de: Brazilian Journal of Medical and Biological Research Ribeirão Preto v. 38, n. 11, p. 1711-1717, 2005
  • Notas Locais: Na FMVZ, ver acervo: SOU-35 Documento Digital
  • Descrição: Altemative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a noninvasive, non-stressor, econornical, and animal saving technique which allows longitudinal studies by perrnitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian harnsters during the estrous cyc1e (control) and in feres of superovulated females. Metabolites were extracted by fecal dilution in ethanol and quantified by solid phase radioimmunoassay. Median estroge;. and progesterone concentrations were 9.703 and 180.74 ng/g feces in the control group, respectively. Peaks of estrogen (22.44:t 4.54 ng/g feces) and progesterone (655.95:t 129.93 ng/g feres) mean fecal concentrations respectivelyoccurred 12 h before and immediately after ovulation, which is easily detected in this species by observation of a characteristic vaginal postovulatory discharge. Median estrogen and progesterone . concentrations (28.159 and 586.57 ng/g feres, respectively) were significantly higher in superovulated animal feces (P <
    0.00(1). The present study demonstrated that it is possible to monitor ovarian activity in Syrian harnsters non-invasively by measuring fecal estradiol and progesterone metabolites. This technique appears to be a quite encouraging method for the development of new endocrinologic studies on laboratory animals.
  • Editor: Ribeirão Preto
  • Data de criação/publicação: 2005
  • Formato: p. 1711-1717.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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