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New highly antigenic linear B cell epitope peptides from PvAMA-1 as potential vaccine candidates

Fantin, Raianna F ; Fraga, Vanessa G ; Lopes, Camila A ; de Azevedo, Isabella C ; Reis-Cunha, João L ; Pereira, Dhelio B ; Lobo, Francisco P ; de Oliveira, Marcela M ; Dos Santos, Anderson C ; Bartholomeu, Daniela C ; Fujiwara, Ricardo T ; Bueno, Lilian L Ho, Paulo Lee

PloS one, 2021-11, Vol.16 (11), p.e0258637-e0258637 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
New highly antigenic linear B cell epitope peptides from PvAMA-1 as potential vaccine candidates
Autor: Fantin, Raianna F ; Fraga, Vanessa G ; Lopes, Camila A ; de Azevedo, Isabella C ; Reis-Cunha, João L ; Pereira, Dhelio B ; Lobo, Francisco P ; de Oliveira, Marcela M ; Dos Santos, Anderson C ; Bartholomeu, Daniela C ; Fujiwara, Ricardo T ; Bueno, Lilian L
Ho, Paulo Lee
Assuntos: Adult ; Amino Acid Sequence ; Amino acids ; Analysis ; Antibody Formation - immunology ; Antigenic determinants ; Antigenicity ; Antigens ; Antigens, Protozoan - immunology ; Biology and Life Sciences ; Care and treatment ; Case-Control Studies ; Depletion ; Development and progression ; Diagnosis ; Epitopes ; Epitopes, B-Lymphocyte - immunology ; Female ; Humans ; Immune response ; Immune system ; Immunodominant Epitopes - immunology ; Immunogenicity ; Immunoglobulin G ; Immunoglobulin G - immunology ; Infections ; Innovations ; Lymphocytes B ; Malaria ; Malaria vaccine ; Malaria Vaccines - immunology ; Malaria, Vivax - epidemiology ; Malaria, Vivax - immunology ; Male ; Markov chains ; Markov processes ; Mass spectrometry ; Mediation ; Medicine ; Medicine and Health Sciences ; Membrane Proteins - immunology ; Middle Aged ; Parasitology ; People and places ; Peptides ; Peptides - chemistry ; Peptides - immunology ; Plasmodium vivax - immunology ; Protein structure ; Protein Structure, Secondary ; Proteins ; Protozoan Proteins - immunology ; Reactivity ; Research and Analysis Methods ; Risk factors ; Scientific imaging ; Secondary structure ; Synthetic peptides ; Testing ; Vaccine development ; Vaccines ; Vector-borne diseases
É parte de: PloS one, 2021-11, Vol.16 (11), p.e0258637-e0258637
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Peptide-based vaccines have demonstrated to be an important way to induce long-lived immune responses and, therefore, a promising strategy in the rational of vaccine development. As to malaria, among the classic vaccine targets, the Apical membrane antigen (AMA-1) was proven to have important B cell epitopes that can induce specific immune response and, hence, became key players for a vaccine approach. The peptides selection was carried out using a bioinformatic approach based on Hidden Markov Models profiles of known antigens and propensity scale methods based on hydrophilicity and secondary structure prediction. The antigenicity of the selected B-cell peptides was assessed by multiple serological assays using sera from acute P.vivax infected subjects. The synthetic peptides were recognized by 45.5%, 48.7% and 32.2% of infected subjects for peptides I, II and III respectively. Moreover, when synthetized together (tripeptide), the reactivity increases up to 62%, which is comparable to the reactivity found against the whole protein PvAMA-1 (57%). Furthermore, IgG reactivity against the tripeptide after depletion was reduced by 42%, indicating that these epitopes may be responsible for a considerable part of the protein immunogenicity. These results represent an excellent perspective regarding future chimeric vaccine constructions that may come to contemplate several targets with the potential to generate the robust and protective immune response that a vivax malaria vaccine needs to succeed.
Editor: United States: Public Library of Science
Idioma: Inglês

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New highly antigenic linear B cell epitope peptides from PvAMA-1 as potential vaccine candidates

Fantin, Raianna F ; Fraga, Vanessa G ; Lopes, Camila A ; de Azevedo, Isabella C ; Reis-Cunha, João L ; Pereira, Dhelio B ; Lobo, Francisco P ; de Oliveira, Marcela M ; Dos Santos, Anderson C ; Bartholomeu, Daniela C ; Fujiwara, Ricardo T ; Bueno, Lilian L Ho, Paulo Lee

United States: Public Library of Science

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