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Minimap2: pairwise alignment for nucleotide sequences
Li, Heng Birol, Inanc
Bioinformatics, 2018-09, Vol.34 (18), p.3094-3100
[Periódico revisado por pares]
England: Oxford University Press
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Título:
Minimap2: pairwise alignment for nucleotide sequences
Autor:
Li, Heng
Birol, Inanc
Assuntos:
Original Papers
É parte de:
Bioinformatics, 2018-09, Vol.34 (18), p.3094-3100
Notas:
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição:
Abstract Motivation Recent advances in sequencing technologies promise ultra-long reads of ∼100 kb in average, full-length mRNA or cDNA reads in high throughput and genomic contigs over 100 Mb in length. Existing alignment programs are unable or inefficient to process such data at scale, which presses for the development of new alignment algorithms. Results Minimap2 is a general-purpose alignment program to map DNA or long mRNA sequences against a large reference database. It works with accurate short reads of ≥100 bp in length, ≥1 kb genomic reads at error rate ∼15%, full-length noisy Direct RNA or cDNA reads and assembly contigs or closely related full chromosomes of hundreds of megabases in length. Minimap2 does split-read alignment, employs concave gap cost for long insertions and deletions and introduces new heuristics to reduce spurious alignments. It is 3-4 times as fast as mainstream short-read mappers at comparable accuracy, and is ≥30 times faster than long-read genomic or cDNA mappers at higher accuracy, surpassing most aligners specialized in one type of alignment. Availability and implementation https://github.com/lh3/minimap2 Supplementary information Supplementary data are available at Bioinformatics online.
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England: Oxford University Press
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Inglês
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