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Salmonella; Reports Outline Salmonella Study Findings from Food and Drug Administration
Obesity, Fitness & Wellness Week, 2012, p.1123
Atlanta: NewsRx
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Título:
Salmonella; Reports Outline Salmonella Study Findings from Food and Drug Administration
Assuntos:
Akiyama
, T
É parte de:
Obesity, Fitness & Wellness Week, 2012, p.1123
Descrição:
Quinolone resistance determinants were examined by sequence analysis of gyrA, gyrB, parC and parE, PCR amplification of qnrA, qnrB and qnrS, and expression of acrB, ramA, marA, soxS and rob using quantitative RT-PCR. The contribution of efflux pump activities to antibiotic resistance was determined by the addition of carbonyl cyanide m-chlorophenylhydrazone (CCCP). The effect of ramR deletion on ciprofloxacin resistance was determined by complementing with wild-type ramR. Salmonella strains 30 and 487 were susceptible to ciprofloxacin and had a single mutation in gyrA as compared with strain 75, which was highly resistant to ciprofloxacin and had a double mutation in gyrA. Increased expression of ramA was associated with high resistance to ciprofloxacin. Strain 75 had a deletion of 315 bp in the ramR gene, which regulates ramA expression. Overexpression of ramA was possibly related to a loss of ramR. Introduction of ramR decreased the MIC of ciprofloxacin from 48 to 24 mg/L.
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Atlanta: NewsRx
Idioma:
Inglês
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