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Characterization of the inflammatory response in syngeneic and allogeneic skin transplantation in BALB/C mice

T Takiishi Luiz Vicente Rizzo; L V Moraes; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Characterization of the inflammatory response in syngeneic and allogeneic skin transplantation in BALB/C mice
  • Autor: T Takiishi
  • Luiz Vicente Rizzo; L V Moraes; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objectives: The process of graft rejection is complex and involves a diversity of populations and mechanisms. The response is triggered by signals due to damage caused by surgery, afterwards the recognition of alloantigens leads to effector mechanisms which increase the inflammatory response and affects the allograft. The role of inflammation in transplantation is still not entirely understood. In this study, we characterize the inflammatory cells that migrate to allogeneic or syngeneic skin grafts after transplantation and investigate if there is an increase of allograft survival when transplanted simultaneously with the syngeneic graft. Methods and Results: BALB/c mice (H-2d) were transplanted with the tail skin from syngeneic or allogeneic donors (NZW;H-2z). Grafts were removed at 24, 72 hours, 7 or 9 days after transplantation and cultured in 500 µL of complete medium for 24 hours. Cells that emigrated from the grafts were characterized by cytospin and flow cytometry. IL-10 and NO production were evaluated in the supernatants. Syngeneic and allogeneic skin grafts were also transplanted simultaneously into recipients and graft survival was evaluated. Results show an increase of total number of emigrant cells during the evaluation period both in syngeneic and allogeneic grafts. The majority of the population was composed of neutrophils at all time-points although an increase of mononuclear cells was observed from day 1 until
    day 7. A higher production of nitric oxide in allogeneic skin was observed in all time-points when compared to syngeneic grafts; IL-10 production was higher in syngeneic graft in the first 24 hours. In receptors of simultaneous transplantation, allograft survival was increased when compared to mice that received allografts only. Conclusion: These data suggest differential regulatory mechanism in allogeneic and syngeneic grafts and that increased allograft survival may be a consequence of the bystander suppression due to syngeneic graft.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. TR.030.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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