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Single-Cell Transcriptome Identifies the Renal Cell Type Tropism of Human BK Polyomavirus

Yang, Feng ; Chen, Xutao ; Zhang, Hui ; Zhao, Guo-Dong ; Yang, Huifei ; Qiu, Jiang ; Meng, Siyan ; Wu, Penghan ; Tao, Liang ; Wang, Qin ; Huang, Gang

International journal of molecular sciences, 2023-01, Vol.24 (2), p.1330 [Peer Reviewed Journal]

Switzerland: MDPI AG

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Title:
Single-Cell Transcriptome Identifies the Renal Cell Type Tropism of Human BK Polyomavirus
Author: Yang, Feng ; Chen, Xutao ; Zhang, Hui ; Zhao, Guo-Dong ; Yang, Huifei ; Qiu, Jiang ; Meng, Siyan ; Wu, Penghan ; Tao, Liang ; Wang, Qin ; Huang, Gang
Subjects: Antiviral Agents ; Biopsy ; BK polyomavirus ; BK Virus - genetics ; Cell interactions ; Cells ; Collecting duct ; Communication ; Endoplasmic reticulum ; Endothelial Cells ; Gene expression ; Genomes ; Gluconeogenesis ; Glycolysis ; Humans ; immune microenvironment ; Immune system ; Infections ; Kidney ; Kidney Transplantation - adverse effects ; Kidneys ; Metabolism ; Microenvironments ; multi-omics ; Polyomavirus Infections - genetics ; Quality control ; Ranking ; single-cell transcriptomics ; Statistical power ; Transcription factors ; Transcriptome ; Transcriptomes ; Transcriptomics ; Tropism
Is Part Of: International journal of molecular sciences, 2023-01, Vol.24 (2), p.1330
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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These authors contributed equally to this work.
Description: BK polyomavirus (BKPyV) infection is the main factor affecting the prognosis of kidney transplant recipients, as no antiviral agent is yet available. A better understanding of the renal-cell-type tropism of BKPyV can serve to develop new treatment strategies. In this study, the single-cell transcriptomic analysis demonstrated that the ranking of BKPyV tropism for the kidney was proximal tubule cells (PT), collecting duct cells (CD), and glomerular endothelial cells (GEC) according to the signature of renal cell type and immune microenvironment. In normal kidneys, we found that BKPyV infection-related transcription factors P65 and CEBPB were PT-specific transcription factors, and PT showed higher glycolysis/gluconeogenesis activities than CD and GEC. Furthermore, in the BKPyV-infected kidneys, the percentage of late viral transcripts in PT was significantly higher than in CD and GEC. In addition, PT had the smallest cell-cell interactions with immune cells compared to CD and GEC in both normal and BKPyV-infected kidneys. Subsequently, we indirectly demonstrated the ranking of BKPyV tropism via the clinical observation of sequential biopsies. Together, our results provided in-depth insights into the renal cell-type tropism of BKPyV in vivo at single-cell resolution and proposed a novel antiviral target.
Publisher: Switzerland: MDPI AG
Language: English

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Single-Cell Transcriptome Identifies the Renal Cell Type Tropism of Human BK Polyomavirus

Yang, Feng ; Chen, Xutao ; Zhang, Hui ; Zhao, Guo-Dong ; Yang, Huifei ; Qiu, Jiang ; Meng, Siyan ; Wu, Penghan ; Tao, Liang ; Wang, Qin ; Huang, Gang

Switzerland: MDPI AG

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