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High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine

Chan, E. C. Y. ; Ho, P. C.

Rapid communications in mass spectrometry, 2000-01, Vol.14 (21), p.1959-1964 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

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Título:
High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine
Autor: Chan, E. C. Y. ; Ho, P. C.
Assuntos: Atmospheric Pressure ; Buffers ; Catecholamines - chemistry ; Catecholamines - urine ; Chromatography, High Pressure Liquid - methods ; Chromatography, Ion Exchange ; Dopamine - analogs & derivatives ; Dopamine - analysis ; Dopamine - chemistry ; Dopamine - urine ; Epinephrine - chemistry ; Epinephrine - urine ; Humans ; Mass Spectrometry - methods ; Metanephrine - chemistry ; Metanephrine - urine ; Molecular Structure ; Norepinephrine - chemistry ; Norepinephrine - urine ; Normetanephrine - chemistry ; Normetanephrine - urine ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity
É parte de: Rapid communications in mass spectrometry, 2000-01, Vol.14 (21), p.1959-1964
Notas: ark:/67375/WNG-D67PF0GD-4
istex:43333848B87A28642406CF944173948485EE1A05
National University of Singapore - No. RP970326
ArticleID:RCM117
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: An assay of norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NE) and metanephrine (MN) based on high‐performance liquid chromatography (HPLC) in combination with atmospheric pressure chemical ionization mass spectrometry (APcI‐MS) is described. The catecholamines and metanephrines were extracted from urine and aqueous samples using Bio‐Rex 70 cation‐exchange resin and subjected to analysis by HPLC/APcI‐MS. The separation was performed on a C18 column in 50 mM ammonium formate buffer, pH 3.0, using a flow rate of 0.8 mL/min. Acetonitrile was added post‐column at a flow rate of 0.2 mL/min via a post‐column addition tee. The total analysis time was 6.5 min. The quantitative analysis was performed using 3,4‐dihydroxybenzylamine (DHBA) as the internal standard (I.S.). Selected ion monitoring detection was applied: m/z 170 (for NE), 184 (for E and NM), 154 (for DA), 198 (for MN) and 140 (for DHBA, I.S.). The limits of quantitation were 5 ng/mL for NE, E and DA and 2.5 ng/mL for NM and MN. The recovery ranged from 75 to 83% for each analyte. The method was found to be simple and highly selective for the determination of catecholamines and metanephrines in the urine of patients suspected of pheochromocytoma. Copyright © 2000 John Wiley & Sons, Ltd.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

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High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine

Chan, E. C. Y. ; Ho, P. C.

Chichester, UK: John Wiley & Sons, Ltd

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