Idioma:

Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors

Anraku, Yasutaka ; Kishimura, Akihiro ; Kamiya, Mako ; Tanaka, Sayaka ; Nomoto, Takahiro ; Toh, Kazuko ; Matsumoto, Yu ; Fukushima, Shigeto ; Sueyoshi, Daiki ; Kano, Mitsunobu R. ; Urano, Yasuteru ; Nishiyama, Nobuhiro ; Kataoka, Kazunori

Angewandte Chemie, 2016-01, Vol.128 (2), p.570-575 [Periódico revisado por pares]

Weinheim: WILEY-VCH Verlag

Texto completo disponível

Citações Citado por

Enviar para

Título:
Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors
Autor: Anraku, Yasutaka ; Kishimura, Akihiro ; Kamiya, Mako ; Tanaka, Sayaka ; Nomoto, Takahiro ; Toh, Kazuko ; Matsumoto, Yu ; Fukushima, Shigeto ; Sueyoshi, Daiki ; Kano, Mitsunobu R. ; Urano, Yasuteru ; Nishiyama, Nobuhiro ; Kataoka, Kazunori
Assuntos: Activation ; Biomedical materials ; Chemical compounds ; Chemistry ; Confocal microscopy ; Construction ; Continuous production ; Crosslinking ; Design engineering ; Drugs ; Enzymatic activity ; Enzyme ; Enzyme activity ; Enzymes ; Fluorescence ; Galactosidase ; In-vivo-Bildgebung ; Lipids ; Mice ; Microscopy ; Nanoreaktoren ; Nanostructure ; Preservation ; Stress concentration ; Therapy ; Tissues ; Tumors ; Vesicles ; Vesikel ; Wirkstofftransport ; β-Galactosidase
É parte de: Angewandte Chemie, 2016-01, Vol.128 (2), p.570-575
Notas: istex:FF6BF79F64362CFF615EE97BD81C329F7A88F7F8
Japan Society for the Promotion of Science (JSPS)
Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan
Japan Science and Technology Agency (JST)
ark:/67375/WNG-9K6B010W-Z
ArticleID:ANGE201508339
Council for Science and Technology Policy (CSTP)
Grant-in-Aid for Scientific Research - No. 23685037; No. 25107709; No. 26288082; No. 24700476
Naito Foundation of Science
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The design and construction of nanoreactors are important for biomedical applications of enzymes, but lipid‐ and polymeric‐vesicle‐based nanoreactors have some practical limitations. We have succeeded in preparing enzyme‐loaded polyion complex vesicles (PICsomes) through a facile protein‐loading method. The preservation of enzyme activity was confirmed even after cross‐linking of the PICsomes. The cross‐linked β‐galactosidase‐loaded PICsomes (β‐gal@PICsomes) selectively accumulated in the tumor tissue of mice. Moreover, a model prodrug, HMDER‐βGal, was successfully converted into a highly fluorescent product, HMDER, at the tumor site, even 4 days after administration of the β‐gal@PICsomes. Intravital confocal microscopy showed continuous production of HMDER and its distribution throughout the tumor tissues. Thus, enzyme‐loaded PICsomes are useful for prodrug activation at the tumor site and could be a versatile platform for enzyme delivery in enzyme prodrug therapy. Wertvolle Fracht: Polyionische komplexe Vesikel (PICsome) wurden durch einfaches Mischen im Vortexer mit Enzymen beladen, die danach ohne Aktivitätsverlust in Tumorgewebe transportiert wurden. Von Bedeutung für künftige Anwendungen in der Therapie und Tumorbildgebung ist, dass die enzymbeladenen PICsome genutzt werden konnten, um an der Tumorstelle ein Modellprodrug in ein hoch fluoreszierendes Produkt umzuwandeln (siehe Bild).
Editor: Weinheim: WILEY-VCH Verlag
Idioma: Inglês;Alemão

Voltar para lista de resultados

Anterior Resultado 2 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors

Anraku, Yasutaka ; Kishimura, Akihiro ; Kamiya, Mako ; Tanaka, Sayaka ; Nomoto, Takahiro ; Toh, Kazuko ; Matsumoto, Yu ; Fukushima, Shigeto ; Sueyoshi, Daiki ; Kano, Mitsunobu R. ; Urano, Yasuteru ; Nishiyama, Nobuhiro ; Kataoka, Kazunori

Weinheim: WILEY-VCH Verlag

Buscando em bases de dados remotas. Favor aguardar.