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Parameter Optimization for Production of Ligninolytic Enzymes Using Agro-industrial Wastes by Response Surface Method

Gassara, Fatma, INRS-ETE, Universite du Quebec, Quebec, Canada ; Brar, Satinder Kaur, INRS-ETE, Universite du Quebec, Quebec, Canada ; Tyagi, R.D., INRS-ETE, Universite du Quebec, Quebec, Canada ; John, Rojan P., INRS-ETE, Universite du Quebec, Quebec, Canada ; Verma, M., Institut de recherche et de development en agroenvironnement inc. (IRDA), Quebec, Canada ; Valero, J.R., INRS-ETE, Universite du Quebec, Quebec, Canada

Biotechnology and Bioprocess Engineering, 2011, 16(2), , pp.343-351 [Periódico revisado por pares]

Heidelberg: The Korean Society for Biotechnology and Bioengineering

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Título:
Parameter Optimization for Production of Ligninolytic Enzymes Using Agro-industrial Wastes by Response Surface Method
Autor: Gassara, Fatma, INRS-ETE, Universite du Quebec, Quebec, Canada ; Brar, Satinder Kaur, INRS-ETE, Universite du Quebec, Quebec, Canada ; Tyagi, R.D., INRS-ETE, Universite du Quebec, Quebec, Canada ; John, Rojan P., INRS-ETE, Universite du Quebec, Quebec, Canada ; Verma, M., Institut de recherche et de development en agroenvironnement inc. (IRDA), Quebec, Canada ; Valero, J.R., INRS-ETE, Universite du Quebec, Quebec, Canada
Assuntos: Agricultural pollution ; Alcohol ; Bioremediation ; Biotechnology ; Breweries ; Chemistry ; Chemistry and Materials Science ; Copper ; ENZIMAS ; ENZYME ; ENZYMES ; Fermentation ; inducers ; Industrial and Production Engineering ; Industrial wastes ; Laccase ; Lignin ; ligninolytic enzyme ; ligninolytic enzymes ; Lip ; Malus ; Manganese peroxidase ; moisture ; Optimization ; Phanerochaete chrysosporium ; Phanerocheate chrysosporium ; Raw materials ; Research Paper ; response surface ; Studies ; Variables ; veratryl alcohol ; Wastes ; 생물공학
É parte de: Biotechnology and Bioprocess Engineering, 2011, 16(2), , pp.343-351
Notas: E21
2012000183
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
G704-000785.2011.16.2.010
Descrição: Lignin and manganese peroxidase (LiP, MnP) and laccase production by Phanerocheate chrysosporium was optimized by response surface methodology for brewery waste and apple pomace. The effect of moisture, copper sulphate, and veratryl alcohol (VA) concentrations on enzyme production was studied. Moisture and VA had significant positive effect on MnP and LiP production and the viability of P. chrysosporium (p less than 0.05) and copper sulphate produced a negative effect. However, moisture and copper sulphate had a significant positive (p less than 0.05) effect on laccase production, but VA had an insignificant positive effect (p less than 0.05). Higher values of MnP, LiP and viability of P. chrysosporium on apple pomace (1287.5 U MnP/gds (units/gram dry substrate), 305 U LiP/gds, and 10.38 Log 10 viability) and brewery waste (792 U MnP/gds and 9.83 Log 10 viability) were obtained with 80% moisture, 3 mmol/kg VA, and 0.5 mmol/kg copper. LiP production in brewery waste (7.87 U/gds) was maximal at 70% moisture, 2 mmol/kg VA, and 1 mmol/kg copper. Higher production of laccase in apple pomace (789 U/gds) and brewery waste (841 U/gds) were obtained with 80% moisture, 3 mmol/kg VA, and 1.5 mmol/kg copper. Thus, moisture along with VA and copper sulphate was pertinent for the production of ligninolytic enzymes and increased cell viability.
Editor: Heidelberg: The Korean Society for Biotechnology and Bioengineering
Idioma: Inglês

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Parameter Optimization for Production of Ligninolytic Enzymes Using Agro-industrial Wastes by Response Surface Method

Heidelberg: The Korean Society for Biotechnology and Bioengineering

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