Idioma:

Comparison of the cellular molecular stress responses after treatments used in bladder cancer

Shackley, D.C. ; Haylett, A. ; Whitehurst, C. ; Betts, C.D. ; O'Flynn, K. ; Clarke, N.W. ; Moore, J.V.

BJU international, 2002-12, Vol.90 (9), p.924-932 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Comparison of the cellular molecular stress responses after treatments used in bladder cancer
Autor: Shackley, D.C. ; Haylett, A. ; Whitehurst, C. ; Betts, C.D. ; O'Flynn, K. ; Clarke, N.W. ; Moore, J.V.
Assuntos: aminolaevulinic acid ; Animals ; Antibiotics, Antineoplastic - therapeutic use ; Blotting, Western ; collagen ; Collagen - metabolism ; Heat-Shock Proteins - metabolism ; HSP47 ; HSP47 Heat-Shock Proteins ; HSP72 ; HSP72 Heat-Shock Proteins ; Hyperthermia, Induced - methods ; Mice ; Mitomycin - therapeutic use ; Photochemotherapy - methods ; photodynamic therapy ; Stress, Physiological - etiology ; Stress, Physiological - metabolism ; Tumor Cells, Cultured ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - therapy
É parte de: BJU international, 2002-12, Vol.90 (9), p.924-932
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Objective To investigate the molecular stress responses related to the quality of recovery of normal tissue after various treatments for bladder cancer, i.e. hyperthermia, ionizing radiation, mitomycin‐C and 5‐aminolaevulinic acid photodynamic therapy (ALA‐PDT). Materials and methods The study focused particularly on intracellular fibroblast levels of heat‐shock protein‐47 (HSP47) and HSP72, which are associated with collagen metabolism and the development of tolerance to repeated treatment, respectively. Iso‐effective treatment doses (50% clonogenic cell survival) of each method were delivered to a 3T6 murine fibroblast model. Intracellular extracts were analysed at 3, 6, 9, 12 and 24 h after treatment, using Western blot analysis to compare the levels of HSP47 and HSP72. Time‐matched treatment and control groups were quantified by comparison with actin and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) expression using appropriate software. Results There were various changes in levels of HSP expression with treatment method; HSP47 levels were significantly higher after hyperthermia and radiation but not with mitomycin‐C or ALA‐PDT. HSP72 levels were significantly higher with all methods except ALA‐PDT. Conclusions Hyperthermia and ionizing radiation are associated with early increases in levels of HSP47 (a marker of collagen metabolism), in contrast to ALA‐PDT and mitomycin‐C. These findings are compatible with clinical findings where fibrosis/scarring is common with the first two but not the last two methods. In addition, all methods except ALA‐PDT are associated with an increase in HSP 72 (a protein associated with cellular tolerance) and this may help to explain, at a cellular level, why resistance to repeated ALA‐PDT treatments does not seem to occur.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 7 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Comparison of the cellular molecular stress responses after treatments used in bladder cancer

Shackley, D.C. ; Haylett, A. ; Whitehurst, C. ; Betts, C.D. ; O'Flynn, K. ; Clarke, N.W. ; Moore, J.V.

Oxford, UK: Blackwell Publishing Ltd

Buscando em bases de dados remotas. Favor aguardar.