skip to main content
Idioma:

Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting

Martins, Luís Miguel Lourenço ; Marques, Andreia Grilo ; Pereira, Luísa Maria Dotti Silva ; Semião-Santos, Saul José ; Bento, Ofélia Pereira

Postȩpy dermatologii i alergologii, 2017-02, Vol.34 (1), p.60-69 [Periódico revisado por pares]

Poland: Termedia Publishing House

Texto completo disponível

Citações Citado por
  • Título:
    Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting
  • Autor: Martins, Luís Miguel Lourenço ; Marques, Andreia Grilo ; Pereira, Luísa Maria Dotti Silva ; Semião-Santos, Saul José ; Bento, Ofélia Pereira
  • Assuntos: allergens ; atopy ; dog ; grass pollen allergens ; immunoblotting ; Original Paper
  • É parte de: Postȩpy dermatologii i alergologii, 2017-02, Vol.34 (1), p.60-69
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Much less is known about grass-pollen allergens to dogs, when compared with humans. Genetic-based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy. Mapping of ( ) and ( ) allergens for grass pollen-sensitized atopic dogs, for better understanding how individual allergograms may influence the response to grass-pollen immunotherapy. To identify and allergoms for dogs, 15 individuals allergic to grass pollen and sensitized to were selected. and proteomes were separated by isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto (PVDF) membranes and allergens were identified by patient sera IgE in Western Blotting (WB). In , 17 allergens were identified from IEF and 11 from 1-D SDS-PAGE, while from , 18 and 6 allergens were identified, respectively. From 2-D SDS-PAGE 13 spots were identified from and 27 from . Several similarities were found between dog and human and sensitization profiles but no relationship between clinical signs and a specific pattern of allergen recognition was observed. Similarities were found in each patient pattern of sensitization between and , also suggesting cross-reactive phenomena. Further molecular epidemiology approach is needed to understand the role of the sensitization pattern in allergen-specific immunotherapy effectiveness in grass-pollen allergic dogs.
  • Editor: Poland: Termedia Publishing House
  • Idioma: Inglês;Polonês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.