skip to main content
Idioma:

Cocaine activates nuclear factor NF-KB in PC 12 cells

L. B. Lepsch Carolina Demarchi Munhoz; Elisa Mitiko Kawamoto; L. S Lima; C. S Planeta; Cristoforo Scavone; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)

Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005

Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental 2005

Item não circula. Consulte sua biblioteca.(Acessar)

  • Título:
    Cocaine activates nuclear factor NF-KB in PC 12 cells
  • Autor: L. B. Lepsch
  • Carolina Demarchi Munhoz; Elisa Mitiko Kawamoto; L. S Lima; C. S Planeta; Cristoforo Scavone; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)
  • Assuntos: FARMACOLOGIA
  • É parte de: Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Objetivo: Drug abuse is associated with changes in brain function and neurodegenerative process, which, for some drugs, have been shown to be associated with the induction of apoptotic cell death. Cocaine is a widely used drug of abuse and a psychostimulant that acts on the central nervous system by blocking dopamine reuptake sites. NF-_B is a transcription factor which plays an important role in the control of the immunity, inflammation and apoptotic response. Our aim was to evaluate if cocaine can modulate NF-_B in PC 12 cells. Métodos e Resultados: PC 12 cells which was cultured in dishes were treated with increasing concentrations of cocaine (0,05; 0,25; 0,5; 0,75; 1,0mM) for 8 hours. Nuclear extracts were isolated and gel mobility shift assay used to measure changes in NF-_B activity. Competition studies with specific and nonspecific unlabeled double-strand oligonucleotide were also performed. Cocaine (0,75 and 1,0 mM) induced a significant induction of NF-_B in PC 12 cells. This activation was dose dependent. Conclusões: Our results suggest that cocaine induces a dose-dependent activation of NF-_B in PC 12 cells.
  • Editor: Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental
  • Data de criação/publicação: 2005
  • Formato: res. 13.105.
  • Idioma: Português
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.