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Immobilization of mycobacterial cells onto silicone – Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol

Claudino, M.J.C. ; Soares, D. ; Van Keulen, F. ; Marques, M.P.C. ; Cabral, J.M.S. ; Fernandes, P.

Bioresource technology, 2008-05, Vol.99 (7), p.2304-2311 [Periódico revisado por pares]

Oxford: Elsevier Ltd

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Título:
Immobilization of mycobacterial cells onto silicone – Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol
Autor: Claudino, M.J.C. ; Soares, D. ; Van Keulen, F. ; Marques, M.P.C. ; Cabral, J.M.S. ; Fernandes, P.
Assuntos: 4-Androstene-3,17-dione ; Androstenedione - biosynthesis ; Bacterial Adhesion ; Biological and medical sciences ; Catalysis ; Cell immobilization ; Chromatography, High Pressure Liquid ; Feasibility Studies ; Fundamental and applied biological sciences. Psychology ; Mycobacterium ; Mycobacterium - growth & development ; Mycobacterium - physiology ; Mycobacterium sp ; Silicone slabs ; Sitosterols - metabolism ; β-Sitosterol
É parte de: Bioresource technology, 2008-05, Vol.99 (7), p.2304-2311
Notas: http://dx.doi.org/10.1016/j.biortech.2007.05.007
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SourceType-Scholarly Journals-1
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Descrição: Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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Immobilization of mycobacterial cells onto silicone – Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol

Claudino, M.J.C. ; Soares, D. ; Van Keulen, F. ; Marques, M.P.C. ; Cabral, J.M.S. ; Fernandes, P.

Oxford: Elsevier Ltd

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