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Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability

Short, Sarah M ; Mongodin, Emmanuel F ; MacLeod, Hannah J ; Talyuli, Octavio A C ; Dimopoulos, George Olson, Ken E.

PLoS neglected tropical diseases, 2017-07, Vol.11 (7), p.e0005677-e0005677 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability
Autor: Short, Sarah M ; Mongodin, Emmanuel F ; MacLeod, Hannah J ; Talyuli, Octavio A C ; Dimopoulos, George
Olson, Ken E.
Assuntos: Abundance ; Aedes - genetics ; Aedes - microbiology ; Aedes aegypti ; Amino acid metabolism ; Amino acids ; Amino Acids - metabolism ; Animals ; Aquatic insects ; Bacteria ; Bacterial Load ; Biodegradation ; Biology and Life Sciences ; Chain branching ; Composition ; Control ; Degradation ; Dengue ; Dengue - virology ; Dengue fever ; Dengue Virus ; Disease transmission ; Female ; Gastrointestinal Tract - microbiology ; Gene expression ; Genes ; Genes, Insect ; Genetic diversity ; Genomics ; Human diseases ; Humans ; Immunology ; Influence ; Insect Vectors - microbiology ; Insects ; Medicine and Health Sciences ; Metabolism ; Microbiological strains ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Midgut ; Mosquitoes ; Nucleic acids ; Pathogens ; Physical Sciences ; Proliferation ; Public health ; Research and Analysis Methods ; Ribonucleic acid ; Risk factors ; RNA ; RNA Interference ; RNA-mediated interference ; Signal Transduction ; Strains ; Strains (organisms) ; Transcription ; Transcriptome ; Transmission ; Tropical diseases ; Variability ; Vector-borne diseases ; Viral diseases ; Viruses ; Yellow fever mosquito
É parte de: PLoS neglected tropical diseases, 2017-07, Vol.11 (7), p.e0005677-e0005677
Notas: new_version
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Current address: Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil
The authors have declared that no competing interests exist.
Descrição: The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81-92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field.
Editor: United States: Public Library of Science
Idioma: Inglês

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Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability

Short, Sarah M ; Mongodin, Emmanuel F ; MacLeod, Hannah J ; Talyuli, Octavio A C ; Dimopoulos, George Olson, Ken E.

United States: Public Library of Science

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