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GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model

Kuradomi, Rafael Y ; Figueiredo, Márcio A ; Lanes, Carlos F. C ; da Rosa, Carlos E ; Almeida, Daniela V ; Maggioni, Rodrigo ; Silva, Maeli D. P ; Marins, Luis F

Transgenic research, 2011-06, Vol.20 (3), p.513-521 [Periódico revisado por pares]

Dordrecht: Dordrecht : Springer Netherlands

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Título:
GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model
Autor: Kuradomi, Rafael Y ; Figueiredo, Márcio A ; Lanes, Carlos F. C ; da Rosa, Carlos E ; Almeida, Daniela V ; Maggioni, Rodrigo ; Silva, Maeli D. P ; Marins, Luis F
Assuntos: Animal Genetics and Genomics ; Animals ; Animals, Genetically Modified - genetics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biotechnology ; Danio rerio ; Disease Models, Animal ; Female ; females ; fish ; Freshwater ; Fundamental and applied biological sciences. Psychology ; gene overexpression ; genes ; Genetic Engineering ; Genetic technics ; Growth Hormone - genetics ; Growth Hormone - metabolism ; Humans ; hypertrophy ; Hypertrophy - genetics ; Hypertrophy - metabolism ; insulin-like growth factor I ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor I - metabolism ; Life Sciences ; Male ; males ; Methods. Procedures. Technologies ; Molecular Medicine ; Muscle Development - genetics ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - pathology ; Original Paper ; Plant Genetics and Genomics ; protein content ; Sex Factors ; skeletal muscle ; Transgenic animals and transgenic plants ; Transgenics ; Up-Regulation ; Zebrafish - genetics ; Zebrafish - metabolism
É parte de: Transgenic research, 2011-06, Vol.20 (3), p.513-521
Notas: http://dx.doi.org/10.1007/s11248-010-9429-y
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Descrição: The aim of the present study was to analyse the morphology of white skeletal muscle in males and females from the GH-transgenic zebrafish (Danio rerio) lineage F0104, comparing the expression of genes related to the somatotrophic axis and myogenesis. Histological analysis demonstrated that transgenic fish presented enhanced muscle hypertrophy when compared to non-transgenic fish, with transgenic females being more hypertrophic than transgenic males. The expression of genes related to muscle growth revealed that transgenic hypertrophy is independent from local induction of insulin-like growth factor 1 gene (igf1). In addition, transgenic males exhibited significant induction of myogenin gene (myog) expression, indicating that myog may mediate hypertrophic growth in zebrafish males overexpressing GH. Induction of the α-actin gene (acta1) in males, independently from transgenesis, also was observed. There were no significant differences in total protein content from the muscle. Our results show that muscle hypertrophy is independent from muscle igf1, and is likely to be a direct effect of excess circulating GH and/or IGF1 in this transgenic zebrafish lineage.
Editor: Dordrecht: Dordrecht : Springer Netherlands
Idioma: Inglês

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GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model

Kuradomi, Rafael Y ; Figueiredo, Márcio A ; Lanes, Carlos F. C ; da Rosa, Carlos E ; Almeida, Daniela V ; Maggioni, Rodrigo ; Silva, Maeli D. P ; Marins, Luis F

Dordrecht: Dordrecht : Springer Netherlands

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