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An inducible surface presentation system improves cellular immunity against human papillomavirus type 16 E7 antigen in mice after nasal administration with recombinant lactococci

Bermudez-Humaran, Luis G ; Cortes-Perez, Naima G ; Le Loir, Yves ; Alcocer-Gonzalez, Juan M ; Tamez-Guerra, Reyes S ; de Oca-Luna, Roberto Montes ; Langella, Philippe

Journal of medical microbiology, 2004-05, Vol.53 (5), p.427-433 [Periódico revisado por pares]

Reading: Soc General Microbiol

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Título:
An inducible surface presentation system improves cellular immunity against human papillomavirus type 16 E7 antigen in mice after nasal administration with recombinant lactococci
Autor: Bermudez-Humaran, Luis G ; Cortes-Perez, Naima G ; Le Loir, Yves ; Alcocer-Gonzalez, Juan M ; Tamez-Guerra, Reyes S ; de Oca-Luna, Roberto Montes ; Langella, Philippe
Assuntos: Administration, Intranasal ; Animals ; Bacteriology ; Biological and medical sciences ; Cancer Vaccines - administration & dosage ; Cancer Vaccines - genetics ; Cancer Vaccines - immunology ; Cell Wall - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Human health and pathology ; Human papillomavirus 16 ; Humans ; Immunity, Cellular ; Immunization ; Infectious diseases ; Interferon-gamma - metabolism ; Interleukin-2 - metabolism ; Lactococcus lactis ; Lactococcus lactis - genetics ; Lactococcus lactis - immunology ; Life Sciences ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Microbiology ; Microbiology and Parasitology ; Miscellaneous ; Oncogene Proteins, Viral - genetics ; Oncogene Proteins, Viral - immunology ; Papillomaviridae - immunology ; Papillomavirus E7 Proteins ; Papillomavirus Infections - immunology ; Papillomavirus Infections - prevention & control ; Promoter Regions, Genetic ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Uterine Cervical Neoplasms - immunology ; Uterine Cervical Neoplasms - prevention & control ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - genetics ; Vaccines, Synthetic - immunology ; Virology
É parte de: Journal of medical microbiology, 2004-05, Vol.53 (5), p.427-433
Notas: ObjectType-Article-2
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Descrição: 1 Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas. Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L., Mexico 2 Unité de Recherches Laitières et de Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France Correspondence Philippe Langella langella{at}jouy.inra.fr Received September 13, 2003 Accepted January 7, 2004 Human papillomavirus type 16 (HPV-16) is the major causative agent of cervical cancer. To date, vaccine strategies against HPV-16 are based on the ability of the E7 oncoprotein to elicit an immune response against this virus. In this study, the use of an inducible or a constitutive system to produce the HPV-16 E7 protein in Lactococcus lactis , a non-pathogenic and non-invasive Gram-positive bacterium, was compared. The highest E7 production was obtained with the inducible system. When mice were immunized intranasally with recombinant lactococci expressing either inducible or constitutive E7, an antigen-specific cellular response (i.e. secretion of IL2 and IFN- cytokines) was evoked and was substantially higher in mice receiving L. lactis expressing E7 with the inducible system. As bacterial antigen location may influence the immune response, recombinant L. lactis strains that produced E7 in three cellular locations, intracellular, secreted or cell-wall-anchored were evaluated. The highest immune response was elicited by administration of L. lactis producing an inducible cell-wall-anchored form of E7 protein. These promising results represent a step towards the development of a new, safe mucosal vector to treat HPV-related cervical cancer. These authors contributed equally to this work. Abbreviations: CxCa, cervical cancer; HPV-16, human papillomavirus type 16; LAB, lactic acid bacteria.
Editor: Reading: Soc General Microbiol
Idioma: Inglês

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An inducible surface presentation system improves cellular immunity against human papillomavirus type 16 E7 antigen in mice after nasal administration with recombinant lactococci

Bermudez-Humaran, Luis G ; Cortes-Perez, Naima G ; Le Loir, Yves ; Alcocer-Gonzalez, Juan M ; Tamez-Guerra, Reyes S ; de Oca-Luna, Roberto Montes ; Langella, Philippe

Reading: Soc General Microbiol

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