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Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells

Ohno, Shin-ichiro ; Takanashi, Masakatsu ; Sudo, Katsuko ; Ueda, Shinobu ; Ishikawa, Akio ; Matsuyama, Nagahisa ; Fujita, Koji ; Mizutani, Takayuki ; Ohgi, Tadaaki ; Ochiya, Takahiro ; Gotoh, Noriko ; Kuroda, Masahiko

Molecular therapy, 2013-01, Vol.21 (1), p.185-191 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells
Autor: Ohno, Shin-ichiro ; Takanashi, Masakatsu ; Sudo, Katsuko ; Ueda, Shinobu ; Ishikawa, Akio ; Matsuyama, Nagahisa ; Fujita, Koji ; Mizutani, Takayuki ; Ohgi, Tadaaki ; Ochiya, Takahiro ; Gotoh, Noriko ; Kuroda, Masahiko
Assuntos: Animals ; Base Sequence ; Blotting, Western ; Breast cancer ; Breast Neoplasms - metabolism ; Breast Neoplasms - therapy ; Cell Line, Tumor ; Coculture Techniques ; Exosomes - metabolism ; Female ; Humans ; Mice ; MicroRNAs - administration & dosage ; Microscopy, Immunoelectron ; Original ; Real-Time Polymerase Chain Reaction ; Receptor, Epidermal Growth Factor - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Small Interfering
É parte de: Molecular therapy, 2013-01, Vol.21 (1), p.185-191
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SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA (miRNA) to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a miRNA to EGFR-expressing xenograft breast cancer tissue in RAG2–/– mice. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells

Ohno, Shin-ichiro ; Takanashi, Masakatsu ; Sudo, Katsuko ; Ueda, Shinobu ; Ishikawa, Akio ; Matsuyama, Nagahisa ; Fujita, Koji ; Mizutani, Takayuki ; Ohgi, Tadaaki ; Ochiya, Takahiro ; Gotoh, Noriko ; Kuroda, Masahiko

United States: Elsevier Inc

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