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Analytical techniques for the study of polyphenol-protein interactions

Poklar Ulrih, Nataša

Critical reviews in food science and nutrition, 2017-07, Vol.57 (10), p.2144-2161 [Periódico revisado por pares]

United States: Taylor & Francis

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Título:
Analytical techniques for the study of polyphenol-protein interactions
Autor: Poklar Ulrih, Nataša
Assuntos: Animals ; Anticarcinogenic Agents - chemistry ; Anticarcinogenic Agents - metabolism ; Antioxidants - chemistry ; Antioxidants - metabolism ; Binding Sites ; Biochemistry - instrumentation ; Biochemistry - methods ; Biochemistry - trends ; Biosensors ; Calorimetry ; Catechin - analogs & derivatives ; Catechin - chemistry ; Catechin - metabolism ; Circular dichroism ; Complex formation ; Computer applications ; Dichroism ; Differential scanning calorimetry ; Electrochemistry ; Flavonoids - chemistry ; Flavonoids - metabolism ; Fluorescence ; Fourier transforms ; Heat measurement ; Humans ; in-situ techniques ; Ligands ; Light diffraction ; Light scattering ; Mass spectrometry ; Mass spectroscopy ; Microscopy ; Models, Molecular ; Molecular Conformation ; Neutron scattering ; NMR ; Nuclear magnetic resonance ; Pharmacology ; Physicochemical properties ; Polyphenol-protein interactions ; Polyphenols ; Polyphenols - chemistry ; Polyphenols - metabolism ; protein aggregation ; Protein interaction ; Protein Interaction Domains and Motifs ; Proteins ; Resonance scattering ; Reviews ; single molecule detection ; Small angle X ray scattering ; the mechanisms of polyphenol-protein interactions ; Titration ; Titration calorimetry ; X-ray diffraction ; X-ray scattering
É parte de: Critical reviews in food science and nutrition, 2017-07, Vol.57 (10), p.2144-2161
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
Descrição: This mini review focuses on advances in biophysical techniques to study polyphenol interactions with proteins. Polyphenols have many beneficial pharmacological properties, as a result of which they have been the subject of intensive studies. The most conventional techniques described here can be divided into three groups: (i) methods used for screening (in-situ methods); (ii) methods used to gain insight into the mechanisms of polyphenol-protein interactions; and (iii) methods used to study protein aggregation and precipitation. All of these methods used to study polyphenol-protein interactions are based on modifications to the physicochemical properties of the polyphenols or proteins after binding/complex formation in solution. To date, numerous review articles have been published in the field of polyphenols. This review will give a brief insight in computational methods and biosensors and cell-based methods, spectroscopic methods including fluorescence emission, UV-vis adsorption, circular dichroism, Fourier transform infrared and mass spectrometry, nuclear magnetic resonance, X-ray diffraction, and light scattering techniques including small-angle X-ray scattering and small-angle neutron scattering, and calorimetric techniques (isothermal titration calorimetry and differential scanning calorimetry), microscopy, the techniques which have been successfully used for polyphenol-protein interactions. At the end the new methods based on single molecule detection with high potential to study polyphenol-protein interactions will be presented. The advantages and disadvantages of each technique will be discussed as well as the thermodynamic, kinetic or structural parameters, which can be obtained. The other relevant biophysical experimental techniques that have proven to be valuable, such electrochemical methods, hydrodynamic techniques and chromatographic techniques will not be described here.
Editor: United States: Taylor & Francis
Idioma: Inglês

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Analytical techniques for the study of polyphenol-protein interactions

Poklar Ulrih, Nataša

United States: Taylor & Francis

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