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Erg K+ currents modulate excitability in mouse mitral/tufted neurons

Hirdes, Wiebke ; Napp, Nora ; Wulfsen, Iris ; Schweizer, Michaela ; Schwarz, Jürgen R. ; Bauer, Christiane K.

Pflügers Archiv, 2009-11, Vol.459 (1), p.55-70 [Periódico revisado por pares]

Berlin/Heidelberg: Springer-Verlag

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Título:
Erg K+ currents modulate excitability in mouse mitral/tufted neurons
Autor: Hirdes, Wiebke ; Napp, Nora ; Wulfsen, Iris ; Schweizer, Michaela ; Schwarz, Jürgen R. ; Bauer, Christiane K.
Assuntos: Action Potentials - drug effects ; Action Potentials - physiology ; Animals ; Animals, Newborn ; Anti-Arrhythmia Agents - pharmacology ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cells, Cultured ; ERG1 Potassium Channel ; Ether-A-Go-Go Potassium Channels - metabolism ; Human Physiology ; Immunohistochemistry ; Ion Channels ; Methoxyhydroxyphenylglycol - analogs & derivatives ; Methoxyhydroxyphenylglycol - pharmacology ; Mice ; Mice, Inbred C57BL ; Molecular Medicine ; Neurons - drug effects ; Neurons - metabolism ; Neurosciences ; Olfactory Bulb - drug effects ; Olfactory Bulb - metabolism ; Patch-Clamp Techniques ; Piperidines - pharmacology ; Pyridines - pharmacology ; Receptors ; Receptors and Transporters ; Receptors, Metabotropic Glutamate - drug effects ; Receptors, Metabotropic Glutamate - metabolism ; Reverse Transcriptase Polymerase Chain Reaction
É parte de: Pflügers Archiv, 2009-11, Vol.459 (1), p.55-70
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Descrição: Different erg (ether-à-go-go-related gene; Kv11) K + channel subunits are expressed throughout the brain. Especially mitral cells of the olfactory bulb are stained intensely by erg1a, erg1b, erg2, and erg3 antibodies. This led us to study the erg current in mitral/tufted (M/T) neurons from mouse olfactory bulb in primary culture. M/T neurons were identified by their morphology and presence of mGluR1 receptors, and RT-PCR demonstrated the expression of all erg subunits in cultured M/T neurons. Using an elevated external K + concentration, a relatively uniform erg current was recorded in the majority of M/T cells and isolated with the erg channel blocker E-4031. With 4-s depolarizations, the erg current started to activate at −65 mV and exhibited half maximal activation at −51 mV. An increase in the external K + concentration resulted in an increase in erg whole-cell conductance. The specific group 1 mGluR agonist, DHPG, which depolarizes mitral cells, reduced erg channel availability. DHPG accelerated erg current deactivation, reduced the maximum current amplitude, and shifted availability and activation curves to more depolarized potentials. A pharmacological block of erg channels depolarized the resting potential of M/T cells and clearly demonstrated the involvement of erg channels in the control of mitral cell excitability.
Editor: Berlin/Heidelberg: Springer-Verlag
Idioma: Inglês

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Erg K+ currents modulate excitability in mouse mitral/tufted neurons

Hirdes, Wiebke ; Napp, Nora ; Wulfsen, Iris ; Schweizer, Michaela ; Schwarz, Jürgen R. ; Bauer, Christiane K.

Berlin/Heidelberg: Springer-Verlag

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