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Sticholysins, pore-forming proteins from a marine anemone can induce maturation of dendritic cells through a TLR4 dependent-pathway

Laborde, Rady J. ; Ishimura, Mayari E. ; Abreu-Butin, Lianne ; Nogueira, Catarina V. ; Grubaugh, Daniel ; Cruz-Leal, Yoelys ; Luzardo, María C. ; Fernández, Audry ; Mesa, Circe ; Pazos, Fabiola ; Álvarez, Carlos ; Alonso, María E. ; Starnbach, Michael N. ; Higgins, Darren E. ; Fernández, Luis E. ; Longo-Maugéri, Ieda M. ; Lanio, María E.

Molecular immunology, 2021-03, Vol.131, p.144-154 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Sticholysins, pore-forming proteins from a marine anemone can induce maturation of dendritic cells through a TLR4 dependent-pathway
Autor: Laborde, Rady J. ; Ishimura, Mayari E. ; Abreu-Butin, Lianne ; Nogueira, Catarina V. ; Grubaugh, Daniel ; Cruz-Leal, Yoelys ; Luzardo, María C. ; Fernández, Audry ; Mesa, Circe ; Pazos, Fabiola ; Álvarez, Carlos ; Alonso, María E. ; Starnbach, Michael N. ; Higgins, Darren E. ; Fernández, Luis E. ; Longo-Maugéri, Ieda M. ; Lanio, María E.
Assuntos: Animals ; Antigens ; CD8-Positive T-Lymphocytes - metabolism ; Cell Differentiation - physiology ; Cells, Cultured ; Cnidarian Venoms - metabolism ; Cross-presentation ; Dendritic cells ; Dendritic Cells - metabolism ; Female ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microbiology ; Myeloid Cells - metabolism ; Organic Chemicals - metabolism ; Pore-forming proteins ; Proteins ; Signal Transduction - physiology ; Sticholysins ; T cells ; TLR ligand ; Toll-Like Receptor 4 - metabolism
É parte de: Molecular immunology, 2021-03, Vol.131, p.144-154
Descrição: •TLR4/MyD88 pathway is relevant for Sts-induced dendritic cells maturation.•The 3D-structure of Sts is important for inducing dendritic cells maturation.•Free Sts mediate Ag cross-presentation in dendritic cells with CD8+ T cell priming.•TLR4 is involved in the CD8+ T cell response induced by StII-carrying liposomes. Sticholysins (Sts) I and II (StI and StII) are pore-forming proteins (PFPs), purified from the Caribbean Sea anemone Stichodactyla helianthus. StII encapsulated into liposomes induces a robust antigen-specific cytotoxic CD8+ T lymphocytes (CTL) response and in its free form the maturation of bone marrow-derived dendritic cells (BM-DCs). It is probable that the latter is partially supporting in part the immunomodulatory effect on the CTL response induced by StII-containing liposomes. In the present work, we demonstrate that the StII’s ability of inducing maturation of BM-DCs is also shared by StI, an isoform of StII. Using heat-denatured Sts we observed a significant reduction in the up-regulation of maturation markers indicating that both PFP’s ability to promote maturation of BM-DCs is dependent on their conformational characteristics. StII-mediated DC maturation was abrogated in BM-DCs from toll-like receptor (TLR) 4 and myeloid differentiation primary response gene 88 (MyD88)-knockout mice but not in cells from TLR2-knockout mice. Furthermore, the antigen-specific CTL response induced by StII-containing liposomes was reduced in TLR4-knockout mice. These results indicate that StII, and probably by extension StI, has the ability to induce maturation of DCs through a TLR4/MyD88-dependent pathway, and that this activation contributes to the CTL response generated by StII-containing liposomes.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Sticholysins, pore-forming proteins from a marine anemone can induce maturation of dendritic cells through a TLR4 dependent-pathway

England: Elsevier Ltd

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