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Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414

Guo, Na ; Sun, Jianan ; Wang, Wei ; Gao, Li ; Liu, Jinbao ; Liu, Zhen ; Xue, Changhu ; Mao, Xiangzhao

Food chemistry, 2019-07, Vol.286, p.696-702 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414
Autor: Guo, Na ; Sun, Jianan ; Wang, Wei ; Gao, Li ; Liu, Jinbao ; Liu, Zhen ; Xue, Changhu ; Mao, Xiangzhao
Assuntos: Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Chitin - analogs & derivatives ; Chitin - metabolism ; Chitosan ; Chitosan - metabolism ; Chitosanase ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; Enzyme Stability ; Escherichia coli - genetics ; GH 46 family ; Glucosamine - metabolism ; Glycoside Hydrolases - genetics ; Glycoside Hydrolases - metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; Molecular Weight ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Streptomyces - enzymology ; Streptomyces - genetics ; Streptomyces albolongus
É parte de: Food chemistry, 2019-07, Vol.286, p.696-702
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SourceType-Scholarly Journals-1
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Descrição: [Display omitted] •A novel recombinant chitosanase (Csn21c) was identified and characterized.•The chitosanase owned good pH stability and thermal stability.•GH family 46 chitosanase (Csn21c) displayed an exo-type cleavage pattern.•This study provided a tool for production of glucosamine from chitosan. A gene encoding chitosanase from Streptomyces albolongus was cloned, sequenced and expressed in Escherichia coli. The novel recombinant enzyme (Csn21c) was purified by Ni-NTA Superflow Column and showed a molecular mass of 29.6 kDa by SDS-PAGE. The enzyme Csn21c showed the optimal activity in 50 mmol/L Tris-HCl buffer, pH 8.0, and 50 °C and it was strongly activated (2-fold) by Mn2+. It belonged to glycoside hydrolase 46 family according to NCBI database (http://www.ncbi.nlm.nih.gov/) and displayed an exo-type cleavage pattern, hydrolyzing chitosan mainly into d-glucosamine (GlcN) and chitobiose ((GlcN)2) as confirmed by TLC and MS analysis. This study demonstrated that Csn21c can be an effective tool to produce abundant glucosamine and chitooligosaccharides (COS) from chitosan.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414

Guo, Na ; Sun, Jianan ; Wang, Wei ; Gao, Li ; Liu, Jinbao ; Liu, Zhen ; Xue, Changhu ; Mao, Xiangzhao

England: Elsevier Ltd

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