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Aneugenic effects of epirubicin in somatic and germinal cells of male mice

Attia, Sabry Mohamed ; Ahmad, Sheikh Fayaz ; Okash, Radwa Mohamed ; Bakheet, Saleh Abdulrahman Shi, Qinghua

PloS one, 2014-10, Vol.9 (10), p.e109942 [Peer Reviewed Journal]

United States: Public Library of Science

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Title:
Aneugenic effects of epirubicin in somatic and germinal cells of male mice
Author: Attia, Sabry Mohamed ; Ahmad, Sheikh Fayaz ; Okash, Radwa Mohamed ; Bakheet, Saleh Abdulrahman
Shi, Qinghua
Subjects: Analysis ; Aneugens - pharmacology ; Aneuploidy ; Animals ; Anthracyclines ; Assaying ; Biology and Life Sciences ; Bone marrow ; Bone Marrow Cells - drug effects ; Cancer ; Cancer therapies ; Cell division ; Chemotherapy ; Chromosomes ; Colchicine ; Delay ; Deoxyribonucleic acid ; DNA ; DNA probes ; Drug dosages ; Epirubicin ; Epirubicin - pharmacology ; Erythrocytes ; Fluorescence ; Fluorescence in situ hybridization ; Germ cells ; In Situ Hybridization, Fluorescence ; Leukemia ; Male ; Medical personnel ; Medicine and Health Sciences ; Meiosis ; Meiosis - drug effects ; Metabolism ; Metabolites ; Mice ; Micronuclei ; Mitomycin ; Mitomycin C ; Pharmacology ; Pharmacy ; Probes ; Prophase ; Somatic cells ; Sperm ; Spermatozoa - drug effects ; Toxicology ; Tumors
Is Part Of: PloS one, 2014-10, Vol.9 (10), p.e109942
Notes: Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: SMA SAB. Performed the experiments: SMA SFA RMO SAB. Analyzed the data: SMA RMO. Contributed reagents/materials/analysis tools: SMA SFA SAB. Wrote the paper: SMA RMO. Software used in analysis: SMA RMO.
Description: The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo. By using the BrdU-incorporation assay, it could be shown that the meiotic delay caused by epirubicin in germ cells was approximately 48 h. Disomic and diploid sperm were shown in epididymal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XY-sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise to the development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical personnel exposed to epirubicin.
Publisher: United States: Public Library of Science
Language: English

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Aneugenic effects of epirubicin in somatic and germinal cells of male mice

Attia, Sabry Mohamed ; Ahmad, Sheikh Fayaz ; Okash, Radwa Mohamed ; Bakheet, Saleh Abdulrahman Shi, Qinghua

United States: Public Library of Science

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