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Comparative proteomic analysis using 2DE-LC-MS/MS reveals the mechanism of Fuzhuan brick tea extract against hepatic fat accumulation in rats with nonalcoholic fatty liver disease

Liu, Zhonghua ; Lin, Yong ; Zhang, Sheng ; Wang, Die ; Liang, Qionglin ; Luo, Guoan

Electrophoresis, 2015-09, Vol.36 (17), p.2002-2016 [Periódico revisado por pares]

Germany: Blackwell Publishing Ltd

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  • Título:
    Comparative proteomic analysis using 2DE-LC-MS/MS reveals the mechanism of Fuzhuan brick tea extract against hepatic fat accumulation in rats with nonalcoholic fatty liver disease
  • Autor: Liu, Zhonghua ; Lin, Yong ; Zhang, Sheng ; Wang, Die ; Liang, Qionglin ; Luo, Guoan
  • Assuntos: Ameliorative mechanism ; Animals ; Brick ; Chromatography, Liquid ; Comparative proteomics ; Diets ; Electrophoresis, Gel, Two-Dimensional - methods ; Fat accumulation ; Fuzhuan brick tea extract (FTE) ; Gene Expression - drug effects ; Liver ; Male ; Metabolic diseases ; Non-alcoholic Fatty Liver Disease - metabolism ; Nonalcoholic fatty liver disease ; Plant Extracts - pharmacology ; Protective Agents - pharmacology ; Proteins ; Proteins - analysis ; Proteins - metabolism ; Proteome - analysis ; Proteome - drug effects ; Proteome - metabolism ; Proteomics ; Rats ; Rats, Sprague-Dawley ; Tandem Mass Spectrometry ; Tea ; Tea - chemistry
  • É parte de: Electrophoresis, 2015-09, Vol.36 (17), p.2002-2016
  • Notas: Key Project of National Technology Innovation System for Tea Industry in the People's Republic of China - No. CARS-23
    Foundation for Innovation Research Team of Ministry of Education - No. IRT0963
    Scientific Research Fund of Hunan Provincial Education Department - No. 12B064
    Figure S1. Flowchart for the preparation of FTE and analysis of major components. Table S1. Analysis of the major chemical compositions of FTE. Table S2. Information on the proteins with at least 1.5-fold altered expression due to HFD induction and/or HFTE treatment.
    Hunan Provincial Natural Science Foundation of China - No. 13JJ4067
    National Natural Science Foundation of China - No. 31100502
    Specialized Research Foundation for the doctorial Program of Higher Education of China - No. 20114320120004
    ark:/67375/WNG-8LK8DDMN-B
    ArticleID:ELPS5544
    istex:EAF903FE8A35C0B09543BF88B5DD1356E7FCA5FC
    These authors contributed equally to this work.
    See the article online to view Figs.
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  • Descrição: Fuzhuan brick tea has received increasing attention in recent years owing to its benefits for nonalcoholic fatty liver disease (NAFLD) and associated metabolic syndrome. For exploring the ameliorative mechanism, the liver proteomes from three groups of rats fed either a normal control diet (NCD), a high fat diet (HFD), or a HFD supplemented with high‐dose Fuzhuan brick tea extract (FTE) (HFD + HFTE) were comprehensively compared by quantitative proteomics using 2DE‐LC‐MS/MS. This is the first study of the effects of tea aqueous extract on the liver proteome of rats suffering from metabolic syndrome. The results showed that 57 proteins displayed more than 1.5‐fold differences in at least one of two comparisons of HFD versus NCD and HFD versus HFD + HFTE due to HFD feeding and FTE treatment, respectively. Of them, over 75% of proteins exhibited a similar tendency of expression in the two comparisons, meaning FTE was able to correct HFD effects on rat livers. By function analyses, an extensive list of proteins was involved in sugar and lipid metabolism. Compared with HFD‐fed rats, the reduced lipogenesis and enhanced β‐oxidation, tricarboxylic acid cycle and respiratory chain in HFD + HFTE‐fed rats, which mainly contributed to ameliorate hepatic fat accumulation and associated NAFLD. Additionally, some putative drug targets were also revealed such as COX2, PGAM1, ACACB, FAS, and ECHS1.
  • Editor: Germany: Blackwell Publishing Ltd
  • Idioma: Inglês
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