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Effects of organic and inorganic selenium supplementation on frozen-thawed ram semen at two cooling periods

Rodrigues, Jéssica Ferreira ; Dacampo, Lucas Dalle Laste ; Bicca, Diogo Ferreira ; Lüdtke, Diogo Seibert ; Brum, Daniela dos Santos ; Cibin, Francielli Weber Santos

Veterinary research communications, 2024-06, Vol.48 (3), p.1367-1377 [Periódico revisado por pares]

Dordrecht: Springer Netherlands

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Título:
Effects of organic and inorganic selenium supplementation on frozen-thawed ram semen at two cooling periods
Autor: Rodrigues, Jéssica Ferreira ; Dacampo, Lucas Dalle Laste ; Bicca, Diogo Ferreira ; Lüdtke, Diogo Seibert ; Brum, Daniela dos Santos ; Cibin, Francielli Weber Santos
Assuntos: Animals ; Biomedical and Life Sciences ; Cooling ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Enzymatic activity ; Freezing ; Glutathione peroxidase ; Life Sciences ; Male ; Motility ; Oxidative stress ; Reactive oxygen species ; Selenium ; Selenium - administration & dosage ; Selenium - pharmacology ; Selenium compounds ; Semen ; Semen - drug effects ; Semen Analysis - veterinary ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sheep ; Sperm ; Sperm Motility - drug effects ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Supplements ; Thiobarbituric acid ; Veterinary Medicine/Veterinary Science ; Yolk ; Zoology
É parte de: Veterinary research communications, 2024-06, Vol.48 (3), p.1367-1377
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The aim of this study was to evaluate the effects of different selenium compounds on the sperm quality of cryopreserved ram semen. Ejaculates from four rams, collected using an artificial vagina heated to 38 °C, were individually evaluated. The approved ejaculates were pooled and diluted (1:1 v:v) in Tris-egg yolk extender (20%, v/v) and separated into two control groups, one cooled for 2 h and the other for 4 h. The pooled ejaculates at the two cooling periods were supplemented with two doses (0.5 and 1 μg/mL) of organic selenium (ORG), and inorganic selenium (SeNa), each. The samples were packed in 0.25 ml straws, at a concentration of 400 × 10 6 sperms/mL and stored in liquid nitrogen. The straws were thawed in a water bath at 37 °C for 20 s, and the samples were subjected to sperm kinetics evaluation by Computer Assisted Semen Analysis software. Sperm membrane integrity, acrosome morphology, and mitochondrial potential were assessed. In addition, oxidative stress markers reactive oxygen species (ROS), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive species (TBARS), and glutathione peroxidase (GPx) enzyme activity) were also evaluated. No significant improvement was observed in the ram semen quality at the two cooling times. Supplementation of the freezing extender with 0.5 μg/mL ORG, subjected to 4 h cooling period, increased the sperm motility when compared with the control group at the same cooling time. In addition, the 0.5 μg/mL SeNa group, under the 2 h cooling period, showed an increase in sperm motility when compared to the control group at the same cooling period. Considering the importance of sperm motility as a fertility parameter, our study indicates that supplementation with ORG and SeNa can help improve the total motility of the cryopreserved ram semen.
Editor: Dordrecht: Springer Netherlands
Idioma: Inglês

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Effects of organic and inorganic selenium supplementation on frozen-thawed ram semen at two cooling periods

Rodrigues, Jéssica Ferreira ; Dacampo, Lucas Dalle Laste ; Bicca, Diogo Ferreira ; Lüdtke, Diogo Seibert ; Brum, Daniela dos Santos ; Cibin, Francielli Weber Santos

Dordrecht: Springer Netherlands

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