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Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene

Lan, J ; Zhang, X.-H ; Wang, Y ; Chen, J ; Han, Y

Journal of applied microbiology, 2008-09, Vol.105 (3), p.644-651 [Periódico revisado por pares]

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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Título:
Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene
Autor: Lan, J ; Zhang, X.-H ; Wang, Y ; Chen, J ; Han, Y
Assuntos: Animals ; Bacterial Typing Techniques - methods ; Biological and medical sciences ; diseased fish ; DNA Gyrase - genetics ; DNA Primers - genetics ; Edwardsiella tarda ; Edwardsiella tarda - genetics ; Edwardsiella tarda - isolation & purification ; Edwardsiella tarda - ultrastructure ; Fish Diseases - microbiology ; Flatfishes - microbiology ; Fundamental and applied biological sciences. Psychology ; Gram-Negative Bacterial Infections - microbiology ; gyrB gene ; identification ; Marine ; Microbiology ; Phylogeny ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; RNA, Ribosomal, 16S - genetics ; Scophthalmus maximus ; Virulence
É parte de: Journal of applied microbiology, 2008-09, Vol.105 (3), p.644-651
Notas: http://dx.doi.org/10.1111/j.1365-2672.2008.03779.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: The aim of this study was to report an unusual Edwardsiella tarda and develop an effective method to identify this bacterium. During the spring and summer of 2006, an epizootic occurred among cultured turbot (Scophthalmus maximus) in Qingdao, China. A gram-negative, rod-shaped bacterium (designated as LTB-4) was isolated from the infected fish, and was proved to be virulent to turbot. Based on the 16S rDNA sequencing and phenotypic tests, the bacterial pathogen was identified as E. tarda. Unlike those commonly described E. tarda strains, no flagellate was observed. Partial gyrB genes were amplified from E. tarda using the universal primers of gyrB genes and sequenced. The polymerase chain reaction (PCR) primers for the gyrB gene were designed specific to E. tarda. It revealed positive amplification of the gyrB fragment in E. tarda, whereas other bacterial species were negative. In addition, the technique enabled the recognition of E. tarda from diseased fish. The isolate was identified as E. tarda without flagellate and an effective method was developed to identify E. tarda based on using the gyrB gene as a taxonomic marker. The unusual E. tarda was first reported in China and the PCR allowed the rapid and sensitive detection of E. tarda.
Editor: Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
Idioma: Inglês

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Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene

Lan, J ; Zhang, X.-H ; Wang, Y ; Chen, J ; Han, Y

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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