Idioma:

Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis

Burger, Eva ; Mendes, Ana Carolina S C ; Bani, Giulia M A C ; Brigagão, Maísa R P L ; Santos, Gérsika B ; Malaquias, Luiz Cosme C ; Chavasco, Jorge Kleber ; Verinaud, Liana M ; de Camargo, Zoilo P ; Hamblin, Michael R ; Sperandio, Felipe F Vinetz, Joseph M.

PLoS neglected tropical diseases, 2015-02, Vol.9 (2), p.e0003541-e0003541 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis
Autor: Burger, Eva ; Mendes, Ana Carolina S C ; Bani, Giulia M A C ; Brigagão, Maísa R P L ; Santos, Gérsika B ; Malaquias, Luiz Cosme C ; Chavasco, Jorge Kleber ; Verinaud, Liana M ; de Camargo, Zoilo P ; Hamblin, Michael R ; Sperandio, Felipe F
Vinetz, Joseph M.
Assuntos: Animals ; Bone Marrow - immunology ; Bone Marrow - radiation effects ; Care and treatment ; Cytokines ; Female ; Femur - microbiology ; Fungal infections ; Fungi ; Immune response ; Immune system ; Immunology ; Laser therapy ; Low-Level Light Therapy - methods ; Medical research ; Medicine, Experimental ; Methods ; Mice ; Mitochondria - metabolism ; Mycoses ; Neutrophils - immunology ; Paracoccidioides - immunology ; Paracoccidioides - radiation effects ; Paracoccidioidomycosis - immunology ; Paracoccidioidomycosis - microbiology ; Paracoccidioidomycosis - therapy ; Proteins ; Reactive Oxygen Species - metabolism
É parte de: PLoS neglected tropical diseases, 2015-02, Vol.9 (2), p.e0003541-e0003541
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: FFS EB MRPLB. Performed the experiments: ACSCM GMACB GBS LCCM JKC. Analyzed the data: FFS EB MRH. Contributed reagents/materials/analysis tools: LMV ZPdC. Wrote the paper: FFS MRH EB.
These authors contributed equally to this work.
The authors have declared that no competing interests exist.
Descrição: Neutrophils (PMN) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis (PCM), which is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). PCM is of major importance, especially in Latin America, and its treatment relies on the use of antifungal drugs. However, the course of treatment is lengthy, leading to side effects and even development of fungal resistance. The goal of the study was to use low-level laser therapy (LLLT) to stimulate PMN to fight Pb in vivo. Swiss mice with subcutaneous air pouches were inoculated with a virulent strain of Pb or fungal cell wall components (Zymosan), and then received LLLT (780 nm; 50 mW; 12.5 J/cm2; 30 seconds per point, giving a total energy of 0.5 J per point) on alternate days at two points on each hind leg. The aim was to reach the bone marrow in the femur with light. Non-irradiated animals were used as controls. The number and viability of the PMN that migrated to the inoculation site was assessed, as well as their ability to synthesize proteins, produce reactive oxygen species (ROS) and their fungicidal activity. The highly pure PMN populations obtained after 10 days of infection were also subsequently cultured in the presence of Pb for trials of protein production, evaluation of mitochondrial activity, ROS production and quantification of viable fungi growth. PMN from mice that received LLLT were more active metabolically, had higher fungicidal activity against Pb in vivo and also in vitro. The kinetics of neutrophil protein production also correlated with a more activated state. LLLT may be a safe and non-invasive approach to deal with PCM infection.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 10 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis

Burger, Eva ; Mendes, Ana Carolina S C ; Bani, Giulia M A C ; Brigagão, Maísa R P L ; Santos, Gérsika B ; Malaquias, Luiz Cosme C ; Chavasco, Jorge Kleber ; Verinaud, Liana M ; de Camargo, Zoilo P ; Hamblin, Michael R ; Sperandio, Felipe F Vinetz, Joseph M.

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.