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Removal of annexin V–positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome: a controlled and randomized trial in unselected males

Romany, Laura, Ph.D ; Garrido, Nicolás, Ph.D ; Motato, Yamileth, Ph.D ; Aparicio, Belén, Ph.D ; Remohí, José, M.D ; Meseguer, Marcos, Ph.D

Fertility and sterility, 2014-12, Vol.102 (6), p.1567-1575.e1 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Removal of annexin V–positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome: a controlled and randomized trial in unselected males
Autor: Romany, Laura, Ph.D ; Garrido, Nicolás, Ph.D ; Motato, Yamileth, Ph.D ; Aparicio, Belén, Ph.D ; Remohí, José, M.D ; Meseguer, Marcos, Ph.D
Assuntos: Adult ; Annexin A5 - metabolism ; Apoptosis ; apoptotic sperm ; Birth Rate ; Cell Separation - methods ; Female ; Fertilization in Vitro ; Humans ; Internal Medicine ; live birth rate ; Magnetic activated cell sorting, MACS ; Magnetics ; Male ; Obstetrics and Gynecology ; Oocyte Donation ; ovum donation ; Phosphatidylserines - metabolism ; Pregnancy ; Sperm Injections, Intracytoplasmic - methods ; Sperm Maturation - physiology ; sperm selection ; Spermatozoa - immunology ; Spermatozoa - pathology
É parte de: Fertility and sterility, 2014-12, Vol.102 (6), p.1567-1575.e1
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-News-1
ObjectType-Feature-3
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Descrição: Objective To determine the effect of removing presumptive apoptotic sperm cells from samples from unselected males by means of magnetic activated cell sorting (MACS) on live-birth delivery rates after intracytoplasmic sperm injection (ICSI) in couples undergoing ovum donation (OD). Design Prospective, randomized, triple-blinded, and controlled study. Setting Private university-affiliated IVF center. Patient(s) A total of 237 infertile couples undergoing ICSI as part of an OD program. Intervention(s) Semen specimens from the control group were prepared by swim-up. Samples from the study group were prepared by swim-up followed by MACS and incubation with annexin V–conjugated microbeads to remove annexin V–positive (AV+) sperm cells. Main Outcome Measure(s) Fertilization rates, morphological features of early embryo development, implantation rates, ongoing pregnancy rates, and live-birth rates. Result(s) Similar results were obtained between groups for all the parameters compared: fertilization rates of 75.3% (95% confidence interval [CI], 71.6–78.9) versus 72.1% (95% CI, 68.6–75.7); percentage of good-quality embryos on day 2 of 53.7% (95% CI, 50.3–57.1) versus 51.8% (95% CI, 48.3–55.3) and on day 3 of 54.2% (95% CI, 50.7–57.6) versus 48.9% (95% CI, 45.3–52.4); implantation rates of 42.2% (95% CI, 33.8–48.1) versus 40.1% (95% CI, 34.8–49.6); positive beta-hCG tests of 63.2% (95% CI, 54.7–71.6) versus 68.6% (95% CI, 60.2–76.9), and live-birth rates of 48.4% (95% CI, 39.6–57.1) versus 56.4% (95% CI, 47.3–65.5) in the MACS versus control group. None of the differences reached statistical significance. Conclusion(s) Applying MACS technology to remove AV+ sperm cells from unselected males does not improve the reproductive outcome of ICSI in OD.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Removal of annexin V–positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome: a controlled and randomized trial in unselected males

Romany, Laura, Ph.D ; Garrido, Nicolás, Ph.D ; Motato, Yamileth, Ph.D ; Aparicio, Belén, Ph.D ; Remohí, José, M.D ; Meseguer, Marcos, Ph.D

United States: Elsevier Inc

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