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Rapid sex determination of a wild passerine species using loop‐mediated isothermal amplification (LAMP)

Koch, Hanna R. ; Blohm‐Sievers, Elke ; Liedvogel, Miriam

Ecology and evolution, 2019-05, Vol.9 (10), p.5849-5858 [Revista revisada por pares]

England: John Wiley & Sons, Inc

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  • Título:
    Rapid sex determination of a wild passerine species using loop‐mediated isothermal amplification (LAMP)
  • Autor: Koch, Hanna R. ; Blohm‐Sievers, Elke ; Liedvogel, Miriam
  • Materias: Birds ; blackcap ; CHD‐W ; CHD‐Z ; Chromosomes ; Deoxyribonucleic acid ; DNA ; DNA helicase ; Ecological monitoring ; Evolutionary biology ; Evolutionary conservation ; Gender ; Genes ; Laboratories ; Loop‐mediated isothermal amplification ; Males ; molecular sexing ; Original Research ; passerine ; Primers ; Sex ; Sex determination ; Sexing ; Species ; Studies ; Sylvia atricapilla
  • Es parte de: Ecology and evolution, 2019-05, Vol.9 (10), p.5849-5858
  • Notas: Funding information
    All relevant data for this study are included in and accessible through this manuscript.
    Data Availability Statement
    Funding was provided by the Max Planck Society through a Max Planck Research Group grant (ML).
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    SourceType-Scholarly Journals-1
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    Data Availability Statement: All relevant data for this study are included in and accessible through this manuscript.
  • Descripción: Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR‐based methods are the most common technique for molecularly sexing birds in the laboratory, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, but importantly also in the field. Herein, we used loop‐mediated isothermal amplification (LAMP) for rapid sex determination of blood samples from juvenile European blackcaps, Sylvia atricapilla, sampled in the wild. We designed LAMP primers unique to S. atricapilla based on the sex chromosome‐specific gene, chromo‐helicase‐DNA‐binding protein (CHD), optimized the primers for laboratory and field application, and then used them to test a subset of wild‐caught juvenile blackcaps of unknown gender at the time of capture. Sex determination results were fast and accurate. The advantages of this technique are that it allows researchers to identify the sex of individual birds within hours of sampling and eliminates the need for direct access to a laboratory if implemented at a remote field site. This work adds to the increasing list of available LAMP primers for different bird species and is a new addition within the Passeriformes order. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR‐based methods are the most common technique for sexing birds, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, and importantly also in the field. Herein, we used loop‐mediated isothermal amplification (LAMP) for rapid sex determination of juvenile European blackcaps, Sylvia atricapilla, which were collected from the wild as part of a larger behavioral ecology and gene expression study investigating the neuronal and molecular architecture underlying migratory behavior.
  • Editor: England: John Wiley & Sons, Inc
  • Idioma: Inglés
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