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Genetic relatedness among Olea europaea L. cultivars estimated by RAPD analysis

Gomes, S ; Martins-Lopes, P ; Lopes, J ; Guedes-Pinto, H

Acta horticulturae, 2012 (949), p.61-66 [Periódico revisado por pares]

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Título:
Genetic relatedness among Olea europaea L. cultivars estimated by RAPD analysis
Autor: Gomes, S ; Martins-Lopes, P ; Lopes, J ; Guedes-Pinto, H
Assuntos: agarose ; cultivars ; DNA ; gels ; genetic distance ; genetic relationships ; genetic variation ; Olea europaea ; olives ; random amplified polymorphic DNA technique
É parte de: Acta horticulturae, 2012 (949), p.61-66
Notas: http://www.actahort.org/
Descrição: Random Amplified Polymorphic DNA (RAPDs) analysis was used to evaluate the genetic diversity and variability present in 41 olive cultivars grown in Trás-os-Montes Region. Eleven RAPD primers were used. After agarose gels analysis, RAPDs banding patterns were transformed into binary data of presence-absence and matrices were processed with NTSYS-PC. Genetic distances between all pair wise combinations of the cultivars were calculated using Jaccard’s coefficient of similarity and dendrograms were constructed by the UPGMA method. All primers produced polymorphic bands. A total of 114 reproducible RAPD bands were observed, of which 100 were polymorphic, accounting for a high percentage (86%) of polymorphism. Five unique bands, able to be converted into Sequence Characterized Amplified Region (SCAR) markers, were obtained. Dendrogram similarity relationships revealed some clustering according to the olive cultivars geographical origin.
Editor: International Society for Horticultural Science
Idioma: Inglês

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