Idioma:

Clostridium alkalicellum sp. nov., an obligately alkaliphilic cellulolytic bacterium from a soda lake in the Baikal region

Zhilina, T N ; Kevbrin, V V ; Turova, T P ; Lysenko, A M ; Kostrikina, N A ; Zavarzin, G A

Microbiology (New York), 2005-09, Vol.74 (5), p.642-566 [Periódico revisado por pares]

Russia (Federation)

Texto completo disponível

Citações Citado por

Enviar para

Título:
Clostridium alkalicellum sp. nov., an obligately alkaliphilic cellulolytic bacterium from a soda lake in the Baikal region
Autor: Zhilina, T N ; Kevbrin, V V ; Turova, T P ; Lysenko, A M ; Kostrikina, N A ; Zavarzin, G A
Assuntos: Anaerobiosis ; Anti-Bacterial Agents - pharmacology ; Base Composition ; Cellulose - metabolism ; Chloramphenicol - pharmacology ; Clostridium - classification ; Clostridium - drug effects ; Clostridium - isolation & purification ; Clostridium - physiology ; Culture Media ; DNA, Bacterial - genetics ; Fermentation ; Fresh Water - microbiology ; Hydrogen-Ion Concentration ; Molecular Sequence Data ; Nitrogenase - metabolism ; Phylogeny ; RNA, Bacterial - analysis ; RNA, Ribosomal, 16S - analysis ; Russia ; Species Specificity ; Substrate Specificity ; Water Microbiology
É parte de: Microbiology (New York), 2005-09, Vol.74 (5), p.642-566
Descrição: The first anaerobic alkaliphilic cellulolytic microorganism has been isolated from the Verkhnee Beloe soda lake (Buryatiya, Russia) with pH 10.2 and a salt content of up to 24 g/l. Five strains were characterized. Strain Z-7026 was chosen as the type strain. The cells of the isolate are gram-positive spore-forming rods. A mucous external capsule is produced. The microorganism is obligately alkaliphilic, growing in a pH range of 8.0-10.2, with an optimum at pH 9.0. Sodium ions and, in carbonate-buffered media, sodium chloride are obligately required. The microorganism is slightly halophilic; it grows at 0.017-0.4 M Na+ with an optimum at 0.15-0.3 M Na+. The metabolism is fermentative and strictly anaerobic. Cellulose, cellobiose, and xylan can be used as growth substrates. Plant and algal debris can be fermented. Lactate, ethanol, acetate, hydrogen, and traces of formate are produced during cellulose or cellobiose fermentation. Yeast extract or vitamins are required for anabolic purposes. The microorganism fixes dinitrogen and is nitrogenase-positive. It is tolerant to up to 48 mM Na2S. Growth is not inhibited by kanamycin or neomycin. Chloramphenicol, streptomycin, penicillin, ampicillin, ampiox, bacillin, novobiocin, and bacitracin suppress growth. The DNA G+C content is 29.9 mol %. According to the nucleotide sequence of its 16S rRNA gene, strain Z-7026 is phylogenetically close to the neutrophilic cellulolytic bacteria Clostridium thermocellum (95.5%), C. aldrichii (94.9%), and Acetivibrio cellulolyticus (94.8%). It is proposed as a new species: Clostridium alkalicellum sp. nov.
Editor: Russia (Federation)
Idioma: Russo;Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 4 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Clostridium alkalicellum sp. nov., an obligately alkaliphilic cellulolytic bacterium from a soda lake in the Baikal region

Zhilina, T N ; Kevbrin, V V ; Turova, T P ; Lysenko, A M ; Kostrikina, N A ; Zavarzin, G A

Russia (Federation)

Buscando em bases de dados remotas. Favor aguardar.