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Lectin from red algae Amansia multifida Lamouroux: Extraction, characterization and anti-inflammatory activity

Mesquita, Jacilane Ximenes ; de Brito, Tarcisio Vieira ; Fontenelle, Thais Pontes Carvalho ; Damasceno, Renan Oliveira Silva ; de Souza, Marcellus Henrique Loiola Ponte ; de Souza Lopes, José Luiz ; Beltramini, Leila Maria ; Barbosa, André Luiz dos Reis ; Freitas, Ana Lúcia Ponte

International journal of biological macromolecules, 2021-02, Vol.170, p.532-539 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Lectin from red algae Amansia multifida Lamouroux: Extraction, characterization and anti-inflammatory activity
Autor: Mesquita, Jacilane Ximenes ; de Brito, Tarcisio Vieira ; Fontenelle, Thais Pontes Carvalho ; Damasceno, Renan Oliveira Silva ; de Souza, Marcellus Henrique Loiola Ponte ; de Souza Lopes, José Luiz ; Beltramini, Leila Maria ; Barbosa, André Luiz dos Reis ; Freitas, Ana Lúcia Ponte
Assuntos: Animals ; Anti-inflammatory activity ; Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - pharmacology ; Carrageenan - pharmacology ; Cell Movement - drug effects ; Cytokines - metabolism ; Edema - drug therapy ; Edema - metabolism ; Inflammation - chemically induced ; Inflammation - drug therapy ; Inflammation - metabolism ; Inflammation Mediators - chemistry ; Inflammation Mediators - pharmacology ; Lectins - chemistry ; Lectins - pharmacology ; Leukocytes - drug effects ; Leukocytes - metabolism ; Mice ; Neutrophils - drug effects ; Neutrophils - metabolism ; Oxidation-Reduction - drug effects ; Oxidative Stress - drug effects ; Peritonitis - drug therapy ; Peritonitis - metabolism ; Peroxidase - metabolism ; Pharmacological properties ; Protein ; Rhodophyta - chemistry
É parte de: International journal of biological macromolecules, 2021-02, Vol.170, p.532-539
Descrição: Seaweed lectins are very promising biotechnological tools that also gain prominence when applied to the pharmacology field. The purpose of the present work was to isolate and characterize lectin from the red algae Amansia multifida and subsequently test it in general inflammation models. The lectin was purified by ion exchange chromatography, characterized with two-dimensional electrophoresis, automated analysis of amino acid sequences and circular dichroism spectroscopy. The pharmacological tests performed were paw edema induced by carrageenan or rapid inflammatory mediators, peritonitis induced by carrageenan and myeloperoxidase leukocyte count assays, glutathione and cytokine concentration. Our results have identified a 30 KDa molecular weight protein that presents a major secondary structure arranged in β-strand elements (~43%). A fragment of 20 amino acid residues was sequenced and presented low identity to the known classes of lectins from marine alga. This lectin was able to modulate inflammatory parameters such as paw edema, leukocyte migration, oxidative stress and proinflammatory cytokines. Thus, the lectin from the seaweed Amansia multifida has evident anti-inflammatory properties because it acts by reducing the formation of edema by modulating the effect of vascular mediators, migration of neutrophils, proinflammatory cytokines and oxidative stress control.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Lectin from red algae Amansia multifida Lamouroux: Extraction, characterization and anti-inflammatory activity

Mesquita, Jacilane Ximenes ; de Brito, Tarcisio Vieira ; Fontenelle, Thais Pontes Carvalho ; Damasceno, Renan Oliveira Silva ; de Souza, Marcellus Henrique Loiola Ponte ; de Souza Lopes, José Luiz ; Beltramini, Leila Maria ; Barbosa, André Luiz dos Reis ; Freitas, Ana Lúcia Ponte

Netherlands: Elsevier B.V

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