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Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids

de Souza, Silvana Sant Anna ; Catta-Preta, Carolina Moura ; Alves, João Marcelo P ; Cavalcanti, Danielle P ; Teixeira, Marta M G ; Camargo, Erney P ; De Souza, Wanderley ; Silva, Rosane ; Motta, Maria Cristina M Yurchenko, Vyacheslav

PloS one, 2017-11, Vol.12 (11), p.e0187516-e0187516 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids
Autor: de Souza, Silvana Sant Anna ; Catta-Preta, Carolina Moura ; Alves, João Marcelo P ; Cavalcanti, Danielle P ; Teixeira, Marta M G ; Camargo, Erney P ; De Souza, Wanderley ; Silva, Rosane ; Motta, Maria Cristina M
Yurchenko, Vyacheslav
Assuntos: Angomonas deanei ; Atomic force microscopy ; Atomic structure ; Bioinformatics ; Biology and Life Sciences ; Cell division ; Cell growth ; Computer and Information Sciences ; Crithidia fasciculata ; Cytology ; Deoxyribonucleic acid ; DNA ; Fibrils ; Genetic aspects ; Genomes ; Histone H1 ; Leishmania ; Medicine and Health Sciences ; Metabolism ; Microscopy ; Mitochondrial DNA ; Phylogenetics ; Phylogeny ; Physiological aspects ; Polymerase chain reaction ; Proteins ; Protozoa ; Research and Analysis Methods ; Respiration ; Strigomonas culicis ; Topology ; Trypanosoma
É parte de: PloS one, 2017-11, Vol.12 (11), p.e0187516-e0187516
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Current address: Centre for Immunology and Infection, Department of Biology, University of York, Heslington, York, United Kingdom
These authors are joint senior authors on this work.
Descrição: In trypanosomatids, the kinetoplast is the portion of the single mitochondrion that is connected to the basal body and contains the kDNA, a network composed by circular and interlocked DNA. The kDNA packing is conducted by Kinetoplast Associated Proteins (KAPs), which are similar to eukaryotic histone H1. In symbiont-harboring trypanosomatids (SHTs) such as Angomonas deanei and Strigomonas culicis, a ß-proteobacterium co-evolves with the host in a mutualistic relationship. The prokaryote confers nutritional benefits to the host and affects its cell structure. Atomic force microscopy showed that the topology of isolated kDNA networks is quite similar in the two SHT species. Ultrastructural analysis using high-resolution microscopy techniques revealed that the DNA fibrils are more compact in the kinetoplast region that faces the basal body and that the presence of the symbiotic bacterium does not interfere with kDNA topology. However, RT-PCR data revealed differences in the expression of KAPs in wild-type protozoa as compared to aposymbiotic cells. Immunolocalization showed that different KAPs present distinct distributions that are coincident in symbiont-bearing and in symbiont-free cells. Although KAP4 and KAP7 are shared by all trypanosomatid species, the expanded repertoire of KAPs in SHTs can be used as phylogenetic markers to distinguish different genera.
Editor: United States: Public Library of Science
Idioma: Inglês

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Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids

de Souza, Silvana Sant Anna ; Catta-Preta, Carolina Moura ; Alves, João Marcelo P ; Cavalcanti, Danielle P ; Teixeira, Marta M G ; Camargo, Erney P ; De Souza, Wanderley ; Silva, Rosane ; Motta, Maria Cristina M Yurchenko, Vyacheslav

United States: Public Library of Science

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