Idioma:

Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish

Aanes, Håvard ; Østrup, Olga ; Andersen, Ingrid S ; Moen, Lars F ; Mathavan, Sinnakaruppan ; Collas, Philippe ; Alestrom, Peter

BMC genomics, 2013-05, Vol.14 (1), p.331-331, Article 331 [Periódico revisado por pares]

England: BioMed Central Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish
Autor: Aanes, Håvard ; Østrup, Olga ; Andersen, Ingrid S ; Moen, Lars F ; Mathavan, Sinnakaruppan ; Collas, Philippe ; Alestrom, Peter
Assuntos: 3' Untranslated Regions - genetics ; Alternative Splicing ; Amino Acid Sequence ; Analysis ; Animals ; Cell cycle ; Cloning ; Danio rerio ; DNA methylation ; Embryos ; Epigenesis, Genetic ; Epigenetic inheritance ; Epigenetics ; Exons - genetics ; Freshwater ; Gene expression ; Gene Expression Profiling ; Genetic aspects ; Genetic Markers - genetics ; Genetic transcription ; Genetics ; Genome - genetics ; Genomes ; Genomics ; Histones - genetics ; Medical research ; Methyltransferases ; Molecular Sequence Annotation ; Molecular Sequence Data ; Mothers ; RNA Isoforms - genetics ; Sequence Analysis, RNA ; Transcription Initiation Site ; Zebra fish ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish Proteins - chemistry ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism ; Zygote - metabolism
É parte de: BMC genomics, 2013-05, Vol.14 (1), p.331-331, Article 331
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Zebrafish embryos are transcriptionally silent until activation of the zygotic genome during the 10th cell cycle. Onset of transcription is followed by cellular and morphological changes involving cell speciation and gastrulation. Previous genome-wide surveys of transcriptional changes only assessed gene expression levels; however, recent studies have shown the necessity to map isoform-specific transcriptional changes. Here, we perform isoform discovery and quantification on transcriptome sequences from before and after zebrafish zygotic genome activation (ZGA). We identify novel isoforms and isoform switches during ZGA for genes related to cell adhesion, pluripotency and DNA methylation. Isoform switching events include alternative splicing and changes in transcriptional start sites and in 3' untranslated regions. New isoforms are identified even for well-characterized genes such as pou5f1, sall4 and dnmt1. Genes involved in cell-cell interactions such as f11r and magi1 display isoform switches with alterations of coding sequences. We also detect over 1000 transcripts that acquire a longer 3' terminal exon when transcribed by the zygote compared to their maternal transcript counterparts. ChIP-sequencing data mapped onto skipped exon events reveal a correlation between histone H3K36 trimethylation peaks and skipped exons, suggesting epigenetic marks being part of alternative splicing regulation. The novel isoforms and isoform switches reported here include regulators of transcriptional, cellular and morphological changes taking place around ZGA. Our data display an array of isoform-related functional changes and represent a valuable resource complementary to existing early embryo transcriptomes.
Editor: England: BioMed Central Ltd
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 6 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish

Aanes, Håvard ; Østrup, Olga ; Andersen, Ingrid S ; Moen, Lars F ; Mathavan, Sinnakaruppan ; Collas, Philippe ; Alestrom, Peter

England: BioMed Central Ltd

Buscando em bases de dados remotas. Favor aguardar.