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Roles of export genes cgmA and lysE for the production of l-arginine and l-citrulline by Corynebacterium glutamicum

Lubitz, Dorit ; Jorge, João M. P. ; Pérez-García, Fernando ; Taniguchi, Hironori ; Wendisch, Volker F.

Applied microbiology and biotechnology, 2016-10, Vol.100 (19), p.8465-8474 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Roles of export genes cgmA and lysE for the production of l-arginine and l-citrulline by Corynebacterium glutamicum
Autor: Lubitz, Dorit ; Jorge, João M. P. ; Pérez-García, Fernando ; Taniguchi, Hironori ; Wendisch, Volker F.
Assuntos: Actinomycetales infections ; Amino Acid Transport Systems, Basic - genetics ; Amino Acid Transport Systems, Basic - metabolism ; Amino acids ; Analysis ; Applied Microbial and Cell Physiology ; Arginine ; Arginine - metabolism ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Citrulline - metabolism ; Corynebacterium glutamicum ; Corynebacterium glutamicum - enzymology ; Corynebacterium glutamicum - genetics ; Corynebacterium glutamicum - metabolism ; E coli ; Engineering ; Escherichia coli ; Experiments ; Exports ; Gene amplification ; Gene Deletion ; Gene Expression ; Genes ; Life Sciences ; Lysine ; Metabolism ; Metabolites ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Plasmids ; Polyamines ; Studies
É parte de: Applied microbiology and biotechnology, 2016-10, Vol.100 (19), p.8465-8474
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: l -arginine is a semi-essential amino acid with application in cosmetic, pharmaceutical, and food industries. Metabolic engineering strategies have been applied for overproduction of l -arginine by Corynebacterium glutamicum . LysE was the only known l -arginine exporter of this bacterium. However, an l -arginine-producing strain carrying a deletion of lysE still accumulated about 10 mM l -arginine in the growth medium. Overexpression of the putative putrescine and cadaverine export permease gene cgmA was shown to compensate for the lack of lysE with regard to l -arginine export. Moreover, plasmid-borne overexpression of cgmA rescued the toxic effect caused by feeding of the dipeptide Arg-Ala to lysE -deficient C. glutamicum and argO -deficient Escherichia coli strains. Deletion of the repressor gene cgmR improved l -arginine titers by 5 %. Production of l -lysine and l -citrulline was not affected by cgmA overexpression. Taken together, CgmA may function as an export system not only for the diamine putrescine and cadaverine but also for l -arginine. The major export system for l -lysine and l -arginine LysE may also play a role in l -citrulline export since production of l -citrulline was reduced when lysE was deleted and improved by 45 % when lysE was overproduced.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Roles of export genes cgmA and lysE for the production of l-arginine and l-citrulline by Corynebacterium glutamicum

Lubitz, Dorit ; Jorge, João M. P. ; Pérez-García, Fernando ; Taniguchi, Hironori ; Wendisch, Volker F.

Berlin/Heidelberg: Springer Berlin Heidelberg

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