Idioma:

Altered Expression of the Cyclin D1 and Retinoblastoma Genes in Human Esophageal Cancer

Jiang, W ; Zhang, Y J ; Kahn, S M ; Hollstein, M C ; Santella, R M ; Lu, S H ; Harris, C C ; Montesano, R ; Weinstein, I B

Proceedings of the National Academy of Sciences - PNAS, 1993-10, Vol.90 (19), p.9026-9030 [Periódico revisado por pares]

Washington, DC: National Academy of Sciences of the United States of America

Texto completo disponível

Citações Citado por

Enviar para

Título:
Altered Expression of the Cyclin D1 and Retinoblastoma Genes in Human Esophageal Cancer
Autor: Jiang, W ; Zhang, Y J ; Kahn, S M ; Hollstein, M C ; Santella, R M ; Lu, S H ; Harris, C C ; Montesano, R ; Weinstein, I B
Assuntos: 550400 - Genetics ; BASIC BIOLOGICAL SCIENCES ; bcl-1 gene ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; Cancer ; Carcinoma ; CARCINOMAS ; Cell cycle ; Cell Line ; Cell lines ; Cyclin D1 ; Cyclins ; Cyclins - analysis ; Cyclins - genetics ; Cyclins - isolation & purification ; Deoxyribonucleic acid ; DISEASES ; DNA ; DNA, Neoplasm - genetics ; DNA, Neoplasm - isolation & purification ; Esophageal Neoplasms - chemistry ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - pathology ; Esophagus ; expression ; Gastroenterology. Liver. Pancreas. Abdomen ; GENE AMPLIFICATION ; Gene Expression ; Gene Expression Regulation, Neoplastic ; GENE MUTATIONS ; GENE REGULATION ; Genes ; Genes, Retinoblastoma ; Humans ; Immunohistochemistry ; man ; Medical research ; Medical sciences ; Molecular Weight ; MUTATIONS ; NEOPLASMS ; Oncogene Proteins - analysis ; Oncogene Proteins - genetics ; Oncogene Proteins - isolation & purification ; oncogenes ; ORGANIC COMPOUNDS ; PROTEINS ; RB protein ; retinoblastoma ; Retinoblastoma genes ; Retinoblastoma Protein - analysis ; Retinoblastoma Protein - genetics ; Retinoblastoma Protein - isolation & purification ; Throat ; Tumor cell line ; Tumor Cells, Cultured ; tumor suppressor genes ; Tumors
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1993-10, Vol.90 (19), p.9026-9030
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: We have examined DNA from four human esophageal carcinoma cell lines and 50 primary esophageal carcinomas obtained from China, Italy, and France for amplification of the cyclin D1 gene. We also examined 36 of these 50 carcinomas for expression of the cyclin D1 and retinoblastoma (RB) proteins by immunohistochemistry. We found a 3-to 10-fold amplification of the cyclin D1 gene in 16 of the 50 (32%) tumors and in two of the four cell lines. Cyclin D1 protein was overexpressed in 12 of 13 tumors and the two cell lines that showed gene amplification when compared to normal controls. Studies on RB protein expression indicated that 6 of the 36 (17%) tumor samples examined and one cell line did not show detectable expression of this protein. The tumors and cell lines that had cyclin D1 gene amplification and overexpression exhibited normal levels of expression of RB protein. By contrast, the tumors and cell line that did not appear to express the RB protein did not show amplification of the cyclin D1 gene and expressed only low levels of the cyclin D1 protein (P = 0.03). These results suggest that the inhibitory effect of RB on cell cycle progression can be abrogated during tumor development either by loss of expression of the RB gene or by increased expression of the cyclin D1 gene.
Editor: Washington, DC: National Academy of Sciences of the United States of America
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Altered Expression of the Cyclin D1 and Retinoblastoma Genes in Human Esophageal Cancer

Jiang, W ; Zhang, Y J ; Kahn, S M ; Hollstein, M C ; Santella, R M ; Lu, S H ; Harris, C C ; Montesano, R ; Weinstein, I B

Washington, DC: National Academy of Sciences of the United States of America

Buscando em bases de dados remotas. Favor aguardar.