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Antibodies to RNA polymerase III in systemic sclerosis detected by ELISA

SANTIAGO, Mittermayer ; BARON, Murray ; HUDSON, Marie ; BURLINGAME, Rufus W ; FRITZLER, Marvin J

Journal of rheumatology, 2007-07, Vol.34 (7), p.1528-1534 [Periódico revisado por pares]

Toronto, ON: The Journal of Rheumatology

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Título:
Antibodies to RNA polymerase III in systemic sclerosis detected by ELISA
Autor: SANTIAGO, Mittermayer ; BARON, Murray ; HUDSON, Marie ; BURLINGAME, Rufus W ; FRITZLER, Marvin J
Assuntos: Antibodies, Antinuclear - blood ; Biological and medical sciences ; Diseases of the osteoarticular system ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Health Status ; Humans ; Inflammatory joint diseases ; Male ; Medical sciences ; Middle Aged ; RNA Polymerase III - immunology ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Scleroderma, Systemic - blood ; Scleroderma, Systemic - diagnosis ; Scleroderma, Systemic - physiopathology ; Sensitivity and Specificity ; Severity of Illness Index
É parte de: Journal of rheumatology, 2007-07, Vol.34 (7), p.1528-1534
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: OBJECTIVE: To determine serological and clinical variables associated with anti-RNA polymerase III (RNAP-III) antibodies in patients with systemic sclerosis (SSc) using a new ELISA method. METHODS: Sera from 242 patients with SSc were collected from 14 Canadian clinics. Control sera were from 287 blood donors, and 42 patients with infectious disease, 30 with rheumatoid arthritis (RA), and 30 with systemic lupus erythematosus (SLE). Antibodies to RNAP-III were detected by an ELISA kit and antibodies to other cellular antigens were identified by indirect immunofluorescence (IIF) on HEp-2 cell substrate, line immunoassay, immunoprecipitation of recombinant protein, and addressable laser bead immunoassay (ALBIA). RESULTS: Anti-RNAP-III antibodies were detected in 47/242 (19.4%) SSc sera, 0% RA and SLE sera, 1/287 blood donor sera, and 2/42 infectious disease sera. Diffuse disease (59.5%) was more common than limited disease (36.1%) in the anti-RNAP-III-positive patients (p = 0.006) and there was an association between the presence of anti-RNAP-III and kidney and joint/tendon involvement, but there was no association with a nucleolar IIF pattern, lung involvement, or other clinical indicators. There was a negative association between the presence of anti-RNAP-III antibodies and anticentromere by IIF (p = 0.00004) and anti-Scl-70 by ALBIA (p = 0.0005) and line immunoassay (p = 0.003), suggesting a virtually exclusive presence of these antibodies in SSc. CONCLUSION: Anti-RNAP-III autoantibodies were found in nearly 20% of SSc patients but in less than 1% of controls, thus detection of this antibody is a useful marker to help diagnose SSc. As well, this antibody has prognostic utility, since it is associated with scleroderma renal crisis and the diffuse cutaneous form of SSc.
Editor: Toronto, ON: The Journal of Rheumatology
Idioma: Inglês

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Antibodies to RNA polymerase III in systemic sclerosis detected by ELISA

SANTIAGO, Mittermayer ; BARON, Murray ; HUDSON, Marie ; BURLINGAME, Rufus W ; FRITZLER, Marvin J

Toronto, ON: The Journal of Rheumatology

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