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Lipidomics from sample preparation to data analysis: a primer

Züllig, Thomas ; Trötzmüller, Martin ; Köfeler, Harald C.

Analytical and bioanalytical chemistry, 2020-04, Vol.412 (10), p.2191-2209 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Lipidomics from sample preparation to data analysis: a primer
Autor: Züllig, Thomas ; Trötzmüller, Martin ; Köfeler, Harald C.
Assuntos: Analysis ; Analytical Chemistry ; Biochemistry ; Cell membranes ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Current Progress in Lipidomics ; Data analysis ; Data processing ; Energy storage ; Food Science ; Information management ; Ionic mobility ; Laboratory Medicine ; Lipids ; Mass spectrometry ; Mass spectroscopy ; Monitoring/Environmental Analysis ; Organic compounds ; Quality assurance ; Review ; Sample preparation ; Scientific imaging ; Solid phases ; Spectroscopy ; Technology assessment
É parte de: Analytical and bioanalytical chemistry, 2020-04, Vol.412 (10), p.2191-2209
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
Published in the topical collection Current Progress in Lipidomics with guest editors Michal Holčapek, Gerhard Liebisch, and Kim Ekroos.
Descrição: Lipids are amongst the most important organic compounds in living organisms, where they serve as building blocks for cellular membranes as well as energy storage and signaling molecules. Lipidomics is the science of the large-scale determination of individual lipid species, and the underlying analytical technology that is used to identify and quantify the lipidome is generally mass spectrometry (MS). This review article provides an overview of the crucial steps in MS-based lipidomics workflows, including sample preparation, either liquid–liquid or solid-phase extraction, derivatization, chromatography, ion-mobility spectrometry, MS, and data processing by various software packages. The associated concepts are discussed from a technical perspective as well as in terms of their application. Furthermore, this article sheds light on recent advances in the technology used in this field and its current limitations. Particular emphasis is placed on data quality assurance and adequate data reporting; some of the most common pitfalls in lipidomics are discussed, along with how to circumvent them.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Lipidomics from sample preparation to data analysis: a primer

Züllig, Thomas ; Trötzmüller, Martin ; Köfeler, Harald C.

Berlin/Heidelberg: Springer Berlin Heidelberg

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